Biomarkers of human gastrointestinal tract regions.

Details

Ressource 1Download: serval:BIB_33685D28C501.P001 (819.75 [Ko])
State: Public
Version: author
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_33685D28C501
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Biomarkers of human gastrointestinal tract regions.
Journal
Mammalian Genome
Author(s)
Comelli E.M., Lariani S., Zwahlen M.C., Fotopoulos G., Holzwarth J.A., Cherbut C., Dorta G., Corthésy-Theulaz I., Grigorov M.
ISSN
1432-1777[electronic]
Publication state
Published
Issued date
2009
Volume
20
Number
8
Pages
516-527
Language
english
Abstract
Dysregulation of intestinal epithelial cell performance is associated with an array of pathologies whose onset mechanisms are incompletely understood. While whole-genomics approaches have been valuable for studying the molecular basis of several intestinal diseases, a thorough analysis of gene expression along the healthy gastrointestinal tract is still lacking. The aim of this study was to map gene expression in gastrointestinal regions of healthy human adults and to implement a procedure for microarray data analysis that would allow its use as a reference when screening for pathological deviations. We analyzed the gene expression signature of antrum, duodenum, jejunum, ileum, and transverse colon biopsies using a biostatistical method based on a multivariate and univariate approach to identify region-selective genes. One hundred sixty-six genes were found responsible for distinguishing the five regions considered. Nineteen had never been described in the GI tract, including a semaphorin probably implicated in pathogen invasion and six novel genes. Moreover, by crossing these genes with those retrieved from an existing data set of gene expression in the intestine of ulcerative colitis and Crohn's disease patients, we identified genes that might be biomarkers of Crohn's and/or ulcerative colitis in ileum and/or colon. These include CLCA4 and SLC26A2, both implicated in ion transport. This study furnishes the first map of gene expression along the healthy human gastrointestinal tract. Furthermore, the approach implemented here, and validated by retrieving known gene profiles, allowed the identification of promising new leads in both healthy and disease states.
Keywords
Intestinal Epithelial-Cells, Inflammatory-Bowel-Disease, Mucin Gene-Expression, Human Gastric-Mucosa, Crohns-Disease, Beta-Microseminoprotein, Enzymatic Sulfation, Family, Tissues, Proteins
Pubmed
Web of science
Open Access
Yes
Create date
27/10/2009 15:07
Last modification date
01/10/2019 7:17
Usage data