Unlabelled iododeoxyuridine increases the rate of uptake of [125I]iododeoxyuridine in human xenografted glioblastomas
Details
Serval ID
serval:BIB_334D043A5DD7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Unlabelled iododeoxyuridine increases the rate of uptake of [125I]iododeoxyuridine in human xenografted glioblastomas
Journal
European Journal of Nuclear Medicine and Molecular Imaging
ISSN
1619-7070 (Print)
Publication state
Published
Issued date
04/2002
Volume
29
Number
4
Pages
499-505
Notes
Comparative Study
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
5-Iodo-2'-deoxyuridine (IdUrd), a thymidine (TdR) analogue, can be radiolabelled with iodine-125, an Auger radiation emitter, to provoke double-strand breaks once incorporated into DNA of cancer cells. We have previously shown that co-incubation of [125I]IdUrd with unlabelled IdUrd provided an additive cytotoxicity in two human glioblastoma cell lines. This observation was unexpectedly correlated with an increase in the rate of DNA incorporation of [125I]IdUrd. Here, we further evaluated the effects of unlabelled IdUrd on the uptake of [125I]IdUrd in vitro and in vivo in mice xenografted with three human glioblastoma lines. The results showed that, in these three glioblastoma lines, unlabelled IdUrd increased the rate of uptake of [125I]IdUrd in vitro by 2- to 4.4-fold and in vivo by 1.5- to 2.8-fold. The rate of uptake of [125I]IdUrd in normal rapidly dividing tissues was also increased by 1.3- to 2.8-fold. TdR completely blocked [125I]IdUrd uptake in tumours and tissues. Analogues of IdUrd, such as deoxyuridine and 5-iodo-1,3-dimethyuracil, did not reproduce the effect of IdUrd on the uptake of [125I]IdUrd, suggesting that it is not related to protection against [125I]IdUrd degradation. It is concluded that combined administration of unlabelled IdUrd may improve the use of radiolabelled IdUrd for cancer diagnosis or therapy.
Keywords
Animals
Cell Cycle/drug effects
DNA, Neoplasm/genetics/*metabolism
Disease Models, Animal
Glioblastoma/*metabolism/pathology
Humans
Idoxuridine/*pharmacology
Iodine Radioisotopes/*pharmacokinetics
Mice
Mice, Nude
Nucleic Acid Synthesis Inhibitors/*pharmacology
Reference Values
Reproducibility of Results
Sensitivity and Specificity
Tissue Distribution
Transplantation, Heterologous
Tumor Cells, Cultured/metabolism
Xenograft Model Antitumor Assays/methods
Pubmed
Web of science
Create date
25/01/2008 11:22
Last modification date
20/08/2019 13:19