Novel Chlamydia species isolated from snakes are temperature-sensitive and exhibit decreased susceptibility to azithromycin.

Details

Serval ID
serval:BIB_33295F4DC436
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Novel Chlamydia species isolated from snakes are temperature-sensitive and exhibit decreased susceptibility to azithromycin.
Journal
Scientific reports
Author(s)
Staub E., Marti H., Biondi R., Levi A., Donati M., Leonard C.A., Ley S.D., Pillonel T., Greub G., Seth-Smith HMB, Borel N.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
04/04/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
5660
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Chlamydia species have recently been recognized as emerging pathogens in snakes. However, isolation of novel snake chlamydiae is critical and their growth characteristics are largely unknown. In this study, two novel chlamydial species are described: Chlamydia serpentis and Chlamydia poikilothermis, isolated after attempts on 23 cloacal and choanal swabs from 18 PCR-positive captive snakes originating from different Swiss snake collections. Isolation success, growth curve and infectivity rates over a 48-hour time period were dependent on temperature (37 °C for C. serpentis, 28 °C for C. poikilothermis). C. serpentis and C. poikilothermis were sensitive to tetracycline and moxifloxacin during evaluation by in vitro antibiotic susceptibility assay but intermediate to resistant (2-4 μg/ml) to azithromycin. Whole genome sequencing of the isolates provided proof of the novel species status, and gives insights into the evolution of these branches of genus Chlamydia.
Keywords
Animals, Anti-Bacterial Agents/therapeutic use, Azithromycin/therapeutic use, Chlamydia/classification, Chlamydia/drug effects, Chlamydia/genetics, Chlamydia Infections/drug therapy, Chlamydia Infections/microbiology, Chlamydia Infections/veterinary, Drug Resistance, Bacterial, Genome, Bacterial, Metagenomics, Phylogeny, Snakes/microbiology, Temperature, Whole Genome Sequencing
Pubmed
Web of science
Open Access
Yes
Create date
14/04/2018 11:03
Last modification date
26/10/2019 5:09
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