The intrinsic circadian clock in podocytes controls glomerular filtration rate.

Details

Serval ID
serval:BIB_331882D26965
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The intrinsic circadian clock in podocytes controls glomerular filtration rate.
Journal
Scientific reports
Author(s)
Ansermet C., Centeno G., Nikolaeva S., Maillard M.P., Pradervand S., Firsov D.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
06/11/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
16089
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Glomerular filtration rate (GFR), or the rate of primary urine formation, is the key indicator of renal function. Studies have demonstrated that GFR exhibits significant circadian rhythmicity and, that these rhythms are disrupted in a number of pathologies. Here, we tested a hypothesis that the circadian rhythm of GFR is driven by intrinsic glomerular circadian clocks. We used mice lacking the circadian clock protein BMAL1 specifically in podocytes, highly specialized glomerular cells critically involved in the process of glomerular filtration (Bmal1 <sup>lox/lox</sup> /Nphs2-rtTA/LC1 or, cKO mice). Circadian transcriptome profiling performed on isolated glomeruli from control and cKO mice revealed that the circadian clock controls expression of multiple genes encoding proteins essential for normal podocyte function. Direct assessment of glomerular filtration by inulin clearance demonstrated that circadian rhythmicity in GFR was lost in cKO mice that displayed an ultradian rhythm of GFR with 12-h periodicity. The disruption of circadian rhythmicity in GFR was paralleled by significant changes in circadian patterns of urinary creatinine, sodium, potassium and water excretion and by alteration in the diurnal pattern of plasma aldosterone levels. Collectively, these results indicate that the intrinsic circadian clock in podocytes participate in circadian rhythmicity of GFR.
Pubmed
Web of science
Open Access
Yes
Create date
07/11/2019 23:21
Last modification date
08/02/2020 7:26
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