After portal branch ligation in rat, nuclear factor kappaB, interleukin-6, signal transducers and activators of transcription 3, c-fos, c-myc, and c-jun are similarly induced in the ligated and nonligated lobes.

Details

Serval ID
serval:BIB_330BAD3108BD
Type
Article: article from journal or magazin.
Collection
Publications
Title
After portal branch ligation in rat, nuclear factor kappaB, interleukin-6, signal transducers and activators of transcription 3, c-fos, c-myc, and c-jun are similarly induced in the ligated and nonligated lobes.
Journal
Hepatology (baltimore, Md.)
Author(s)
Stärkel P., Horsmans Y., Sempoux C., De Saeger C., Wary J., Lause P., Maiter D., Lambotte L.
ISSN
0270-9139 (Print)
ISSN-L
0270-9139
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
29
Number
5
Pages
1463-1470
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Several studies have emphasized the involvement of transcription factors, cytokines, and proto-oncogenes in initiating the regenerative process after partial hepatectomy. To assess whether these events do specifically occur in a cellular system undergoing regeneration, we studied the induction of nuclear factor kappaB (NFkappaB), interleukin-6 (IL-6), signal transducers and activators of transcription 3 (Stat3), c-fos, c-myc, c-jun, after portal branch ligation (PBL), which produces atrophy of the deprived lobes (70% of the liver parenchyma), whereas the perfused lobes undergo compensatory regeneration. Nuclear extracts and total RNA were prepared from control livers as well as from atrophying and regenerating lobes at 0.5, 1, 2, 5, and 8 after PBL. NFkappaB and Stat3 induction were studied by electrophoretic mobility shift assays and Western blotting. IL-6 and proto-oncogenes expressions were assessed by reverse transcription polymerase chain reaction and Northern blotting, respectively. Assays were also performed after a sham operation. NFkappaB and Stat3 protein expression and DNA binding were rapidly and similarly induced in nuclear extracts from the atrophying and regenerating lobes. IL-6 was elevated in both lobes from 1 to 8 hours after PBL as well as c-fos, c-myc, and c-jun during the first 2 hours. IL-6 and Stat3 but not NFkappaB were also elevated after a sham operation. These findings suggest that the cellular and molecular changes occurring early in a regenerating liver are nonspecific, possibly stress-induced, cellular responses. They do not indicate the future evolution towards atrophy or regeneration.
Keywords
Animals, Blotting, Western, DNA-Binding Proteins/metabolism, Electrophoresis, Interleukin-6/metabolism, Ligation, Liver/metabolism, Male, NF-kappa B/metabolism, Portal Vein/physiology, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-fos/genetics, Proto-Oncogene Proteins c-fos/metabolism, Proto-Oncogene Proteins c-jun/genetics, Proto-Oncogene Proteins c-jun/metabolism, RNA, Messenger/metabolism, Rats, Rats, Wistar, STAT3 Transcription Factor, Trans-Activators/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
20/10/2016 16:37
Last modification date
20/08/2019 13:18
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