Expression of nitric oxide synthase III in human thyroid follicular cells: evidence for increased expression in hyperthyroidism.

Details

Serval ID
serval:BIB_32B99510D74D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of nitric oxide synthase III in human thyroid follicular cells: evidence for increased expression in hyperthyroidism.
Journal
European journal of endocrinology
Author(s)
Colin I.M., Kopp P., Zbären J., Häberli A., Grizzle W.E., Jameson J.L.
ISSN
0804-4643 (Print)
ISSN-L
0804-4643
Publication state
Published
Issued date
06/1997
Peer-reviewed
Oui
Volume
136
Number
6
Pages
649-655
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Nitric oxide mediates a wide array of cellular functions in many tissues. It is generated by three known isoforms of nitric oxide synthases (NOS). Recently, the endothelial isoform, NOSIII, was shown to be abundantly expressed in the rat thyroid gland and its expression increased in goitrous glands. In this study, we analyzed whether NOSIII is expressed in human thyroid tissue and whether levels of expression vary in different states of thyroid gland function. Semiquantitative RT-PCR was used to assess variations in NOSIII gene expression in seven patients with Graves' disease, one with a TSH-receptor germline mutation and six hypothyroid patients (Hashimoto's thyroiditis). Protein expression and subcellular localization were determined by immunohistochemistry (two normal thyroids, five multinodular goiters, ten hyperthyroid patients and two hypothyroid patients). NOSIII mRNA was detected in all samples: the levels were significantly higher in tissues from hyperthyroid patients compared with euthyroid and hypothyroid patients. NOSIII immunoreactivity was detected in vascular endothelial cells, but was also found in thyroid follicular cells. In patients with Graves' disease, the immunostaining was diffusely enhanced in all follicular cells. A more intense signal was observed in toxic adenomas and in samples obtained from a patient with severe hyperthyroidism due to an activating mutation in the TSH receptor. In multinodular goiters, large follicles displayed a weak signal whereas small proliferative follicles showed intense immunoreactivity near the apical plasma membrane. In hypothyroid patients, NOSIII immunoreactivity was barely detectable. In summary, NOSIII is expressed both in endothelial cells and thyroid follicular cells. The endothelial localization of NOSIII is consistent with a role for nitric oxide in the vascular control of the thyroid. NOSIII expression in thyroid follicular cells and the variations in its immunoreactivity suggest a possible role for nitric oxide in thyrocyte function and/or growth.
Keywords
Humans, Hyperthyroidism/enzymology, Hyperthyroidism/pathology, Immunohistochemistry, Isoenzymes/genetics, Isoenzymes/metabolism, Nitric Oxide Synthase/genetics, Nitric Oxide Synthase/metabolism, Polymerase Chain Reaction, RNA, Messenger/metabolism, Reference Values, Thyroid Diseases/enzymology, Thyroid Gland/enzymology, Thyroid Gland/pathology, Transcription, Genetic
Pubmed
Web of science
Create date
30/12/2020 15:38
Last modification date
31/12/2020 6:26
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