Long-term results of tissue development and cell differentiation on Dacron prostheses seeded with microvascular cells in dogs
Details
Serval ID
serval:BIB_329D58EF3692
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Long-term results of tissue development and cell differentiation on Dacron prostheses seeded with microvascular cells in dogs
Journal
Journal of Vascular Surgery
ISSN
0741-5214
Publication state
Published
Issued date
12/1993
Peer-reviewed
Oui
Volume
18
Number
6
Pages
1019-28
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
PURPOSE: The purpose of this study was to investigate in vivo the long-term development, differentiation, and proliferation of the subendothelial tissue on Dacron prostheses seeded with microvascular cells (MVC). METHODS: Autologous MVC from omental adipose tissue were seeded on 4 mm Dacron prostheses and the prostheses interposed in the carotid arteries of mongrel dogs for 5, 13, and 26 weeks. RESULTS: Light and electron microscopic evaluation of patent seeded prostheses demonstrated an almost complete monolayer of endothelial cells and well-organized subendothelial tissue, whereas patent control prostheses were mainly covered by red and white thrombi, which were partially replaced by organized tissue with increased implantation time. The measurements of the thickness of the luminal cell layer in seeded and control grafts showed no statistically significant increase between 5 and 26 weeks of implantation. The subendothelial tissue of seeded prostheses demonstrated a time-dependent maturation of highly synthesizing myofibroblasts embedded in a collagen matrix to cells with features of smooth muscle cells located in a collagen-elastin matrix. In control grafts examined after 26 weeks the spontaneous endothelialization was accompanied by a delayed or incomplete maturation of subendothelial tissue. CONCLUSIONS: Our study indicates that MVC seeded onto Dacron prostheses are able to generate a vascular wall that does not continue to proliferate after prolonged implantation and that increasingly resembles the wall of a normal artery in cell differentiation and intercellular matrix.
Keywords
Animals
*Blood Vessel Prosthesis
Capillary Permeability
Carotid Arteries/*cytology/surgery
Cell Differentiation
Cell Division
Dogs
Endothelium, Vascular/*cytology/transplantation/ultrastructure
Microscopy, Electron, Scanning
Microscopy, Electron, Scanning Transmission
*Polyethylene Terephthalates
Surface Properties
Time Factors
Pubmed
Web of science
Create date
14/02/2008 15:17
Last modification date
20/08/2019 14:18