Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats.

Details

Serval ID
serval:BIB_31F72FEDCF97
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats.
Journal
The Journal of nutrition
Author(s)
Kovačević S., Elaković I., Vojnović Milutinović D., Nikolić-Kokić A., Blagojević D., Matić G., Tappy L., Djordjevic A., Brkljačić J.
ISSN
1541-6100 (Electronic)
ISSN-L
0022-3166
Publication state
Published
Issued date
03/12/2021
Peer-reviewed
Oui
Volume
151
Number
12
Pages
3661-3670
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Both fructose consumption and chronic stress contribute to the development of metabolic disorders. The consequences of such combination are not fully understood.
We investigated whether fructose supplementation and chronic stress synergistically disturb hepatic lipid and glucose metabolism. The role of energy sensing, redox, and inflammatory status during development of metabolic disturbances was investigated.
Female Wistar rats, aged 2.5 mo, were divided into 4 experimental groups: control (C) fed a standard diet (commercial food and drinking water); fructose (F) fed the same food and 10% fructose solution; stress (S) fed the standard diet and subjected to chronic unpredictable stress and, stress + fructose (SF) combining conditions F and S as above. Stress included daily stressors: cold water forced swimming, physical restraint, cold room, wet bedding, rocking, switching, or tilting cages. After 9 wk, hepatic enzymes and transcription factors involved in gluconeogenesis, lipogenesis, fatty acid oxidation, antioxidative defence, energy sensing, and cytokines were assessed by qPCR, Western blotting, and spectrophotometry and analyzed by 2-factor ANOVA.
Fructose increased AMP-activated protein kinase (AMPK) phosphorylation (40%; P < 0.05) and the ratio of inhibitory phosphorylation to total acetyl-CoA carboxylase (46%; P < 0.01), and decreased sterol regulatory element binding protein 1c nuclear translocation by 30% (P < 0.05) in F and SF compared with C rats. Increased phosPck (phoenolpyruvate carboxykinase) (85%) and G6pase(glucose-6-phosphatase) (55%) was observed in S rats (P < 0.05). A 40% decrease in Apob (apolipoprotein B-100) and an increase in hepatic lipids (P < 0.05), together with a double increase in TNF-α (P < 0.001), were observed in S rats, but without liver histopathological changes. These stress effects on lipid accumulation and TNF-α were abolished in SF rats (P < 0.05).
Fructose does not enhance stress effects on hepatic lipid and glucose metabolism but attenuates its effects on hepatic lipid accumulation and inflammation, suggesting that, in female rats, AMPK activation prevails over stress-induced effects.
Keywords
Animals, Diet, Female, Fructose/adverse effects, Fructose/metabolism, Lipogenesis, Liver/metabolism, Rats, Rats, Wistar, AMPK, lipogenesis, low-grade inflammation, oxidative stress, sex differences
Pubmed
Web of science
Open Access
Yes
Create date
21/09/2021 11:53
Last modification date
06/02/2024 8:18
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