Recording from Fly Taste Sensilla.
Details
Serval ID
serval:BIB_31D68AC1AD4A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Recording from Fly Taste Sensilla.
Journal
Cold Spring Harbor protocols
ISSN
1559-6095 (Electronic)
ISSN-L
1559-6095
Publication state
Published
Issued date
03/04/2023
Peer-reviewed
Oui
Volume
2023
Number
4
Pages
pdb.prot108064
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
Gustatory systems sense chemicals upon contact and provide a model to investigate how these stimuli are encoded to inform various behavioral decisions including choice of foods, egg-laying sites, and mating partners. Multiple organs in the body house peripheral gustatory sensory neurons, the axons of which project to discrete regions in the subesophageal zone and ventral ganglion, representing both the location and quality of the taste stimulus. Taste neurons are broadly divided into subpopulations associated with either positive or negative behavioral valence, each expressing combinations of taste receptors-in some cases, more than 30 receptors-encoded by one or more chemosensory gene families that together determine their chemical response properties. Drosophila provides a powerful model to study gustatory coding because a majority of the taste sensory units (sensilla) are present in external taste organs (labellum and legs) and are accessible for electrophysiological analysis of tastant-evoked responses. Moreover, a large body of work on the basic characteristics of individual taste neurons housed in a sensillum, as well as on functional surveys of entire taste organs, provides a foundation for investigating further questions about taste coding, adaptability, and evolution. This protocol describes how to perform recordings of stimulus-evoked activity from Drosophila taste sensilla covering the basics of setting up the electrophysiology rig and stimulus-delivery device, sample preparation, and performing and analyzing the recordings.
Keywords
Animals, Sensilla, Taste/genetics, Drosophila, Sense Organs/physiology, Neurons
Pubmed
Create date
30/11/2022 10:04
Last modification date
12/04/2023 6:54