Antibodies to core lipopolysaccharide determinants: absence of cross-reactivity with heterologous lipopolysaccharides

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Serval ID
serval:BIB_3180292BE6B2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antibodies to core lipopolysaccharide determinants: absence of cross-reactivity with heterologous lipopolysaccharides
Journal
Journal of Infectious Diseases
Author(s)
Heumann  D., Baumgartner  J. D., Jacot-Guillarmod  H., Glauser  M. P.
ISSN
0022-1899
Publication state
Published
Issued date
04/1991
Peer-reviewed
Oui
Volume
163
Number
4
Pages
762-8
Language
english
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
Using monoclonal antibodies directed against defined epitopes of endotoxin core, this study demonstrated that the presentation of lipopolysaccharide (LPS) to antibodies is critical for measuring the specific binding of antibodies to LPS structures. False cross-reactive reactions apparently were observed when free core LPS or lipid A were used as antigens in ELISA, whereas coating with complexes of high-density lipoproteins with core LPS increased both the sensitivity and the specificity of the test compared with coating with free core LPS, so that nonspecific binding of antibodies was largely avoided. Using this technique, it was not possible to find broadly cross-reactive core LPS antibodies after immunization of rabbits and humans with rough mutants of gram-negative bacteria. These observations underscore the need for careful evaluation of the potential for cross-reactivity of antisera and of monoclonal antibodies directed against endotoxin core.
Keywords
Animals Antibodies, Bacterial/*immunology Antibodies, Monoclonal/immunology Cross Reactions Enzyme-Linked Immunosorbent Assay Epitopes/immunology Escherichia coli/*immunology Humans Immune Sera/immunology Immunization Immunoglobulin G/immunology Immunoglobulin M/immunology Lipopolysaccharides/*immunology Lipoproteins, HDL/immunology Rabbits Salmonella/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
19/01/2008 19:12
Last modification date
01/10/2019 7:17
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