In vitro release of endogenous excitatory sulfur-containing amino acids from various rat brain regions.

Details

Serval ID
serval:BIB_3170452F6D73
Type
Article: article from journal or magazin.
Collection
Publications
Title
In vitro release of endogenous excitatory sulfur-containing amino acids from various rat brain regions.
Journal
Journal of Neurochemistry
Author(s)
Do K.Q., Mattenberger M., Streit P., Cuénod M.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Publication state
Published
Issued date
1986
Peer-reviewed
Oui
Volume
46
Number
3
Pages
779-786
Language
english
Notes
Publication types: Comparative Study ; In Vitro ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Efflux of various amino acids from rat brain slices was determined under resting or depolarizing conditions. Slices of neocortex, hippocampus, striatum, cerebellum, mesodiencephalon, pons-medulla, and spinal cord were depolarized by K+ (50 mM) or veratrine (33 micrograms/ml). The 4-N,N-dimethylamino-azobenzene-4'-isothiocyanate (DABITC) derivatization method of Chang [Biochem. J. 199, 537-545 (1981)] for HPLC was adapted for analysis of amino acids and peptides in superfusion solutions. It allowed the separation and simultaneous detection of the sulfur-containing amino acids cysteine sulfinic acid (CSA), cysteic acid (CA), homocysteine sulfinic acid (HCSA), and homocysteic acid (HCA) at the picomole level. All four were shown to be released on depolarization in a Ca2+-dependent manner from brain slices. CSA and HCSA were released from cortex, hippocampus, mesodiencephalon, and, for HCSA only, striatum. HCA release, observed in all regions, was most prominent in cortex and hippocampus. CA was slightly increased by depolarization in hippocampus and mesodiencephalon. These sulfur-containing amino acids have been shown to exert an excitatory action on CNS neurons. The fact that these sulfur-containing amino acids are released as endogenous substances from nervous tissue supports the hypothesis that they play a role in CNS neurotransmission.
Keywords
Amino Acids, Sulfur/metabolism, Animals, Brain/drug effects, Brain/metabolism, Cysteic Acid/metabolism, Cysteine/analogs & derivatives, Cysteine/metabolism, Homocysteine/analogs & derivatives, Homocysteine/metabolism, Male, Neurotransmitter Agents, Potassium/pharmacology, Rats, Tissue Distribution, Veratrine/pharmacology
Pubmed
Create date
06/03/2014 15:59
Last modification date
20/08/2019 13:16
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