Analysis of the S. pombe signalling scaffold protein Cdc11p reveals an essential role for the N-terminal domain in SIN signalling.

Details

Serval ID
serval:BIB_31704
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of the S. pombe signalling scaffold protein Cdc11p reveals an essential role for the N-terminal domain in SIN signalling.
Journal
FEBS Letters
Author(s)
Krapp A., Cano E., Simanis V.
ISSN
0014-5793
Publication state
Published
Issued date
2004
Volume
565
Number
1-3
Pages
176-180
Language
english
Notes
Publication types: Journal Article
Abstract
The initiation of cytokinesis in the fission yeast Schizosaccharomyces pombe is signalled by the septation initiation network (SIN). Signalling originates from the spindle pole body (SPB), where SIN proteins are anchored by a scaffold composed of cdc11p and sid4p. Cdc11p links the other SIN proteins to sid4p and the SPB. Homologues of cdc11p have been identified in Saccharomyes cerevisiae (Nud1p) and human cells (Centriolin). We have defined functional domains of cdc11p by analysis of deletion mutants. We demonstrate that the C-terminal end of cdc11p is necessary for SPB localisation. We also show that the N-terminal domain is necessary and sufficient for signal transduction, since tethering of this domain to the SPB will substitute for cdc11p in SIN function.
Keywords
Cell Cycle Proteins/chemistry, Cell Cycle Proteins/physiology, Cell Division, Cytoskeletal Proteins/chemistry, Cytoskeletal Proteins/physiology, Deoxyribonucleases/metabolism, Drosophila Proteins/metabolism, Gene Deletion, Green Fluorescent Proteins, Luminescent Proteins/metabolism, Mitotic Spindle Apparatus, Mutation, Phosphorylation, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases/metabolism, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins/chemistry, Saccharomyces cerevisiae Proteins/metabolism, Schizosaccharomyces/metabolism, Signal Transduction, Temperature
Pubmed
Web of science
Create date
19/11/2007 12:30
Last modification date
20/08/2019 13:16
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