Identification of corticosteroid-regulated genes in cardiomyocytes by serial analysis of gene expression.

Details

Serval ID
serval:BIB_316B3A979574
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of corticosteroid-regulated genes in cardiomyocytes by serial analysis of gene expression.
Journal
Genomics
Author(s)
Muller O., Pradervand S., Berger S., Centeno G., Milet A., Nicod P., Pedrazzini T., Tronche F., Schütz G., Chien K., Rossier B.C., Firsov D.
ISSN
0888-7543 (Print)
ISSN-L
0888-7543
Publication state
Published
Issued date
2007
Volume
89
Number
3
Pages
370-377
Language
english
Abstract
Corticosteroids (aldosterone, cortisol/corticosterone) exert direct functional effects on cardiomyocytes. However, gene networks activated by corticosteroids in cardiomyocytes, as well as the involvement of the mineralocorticoid receptor (MR) vs the glucocorticoid receptor (GR) in these effects, remain largely unknown. Here we characterized the corticosteroid-dependent transcriptome in primary culture of neonatal mouse cardiomyocytes treated with 10(-6) M aldosterone, a concentration predicted to occupy both MR and GR. Serial analysis of gene expression revealed 101 aldosterone-regulated genes. The MR/GR specificity was characterized for one regulated transcript, namely ecto-ADP-ribosyltransferase-3 (Art3). Using cardiomyocytes from GR(null/null) or MR(null/null) mice we demonstrate that in GR(null/null) cardiomyocytes the response is abrogated, but it is fully maintained in MR(null/null) cardiomyocytes. We conclude that Art3 expression is regulated exclusively via the GR. Our study identifies a new set of corticosteroid-regulated genes in cardiomyocytes and demonstrates a new approach to studying the selectivity of MR- vs GR-dependent effects.
Keywords
ADP Ribose Transferases/genetics, Aldosterone/metabolism, Animals, Animals, Newborn, Cells, Cultured, GPI-Linked Proteins, Gene Expression Profiling, Gene Expression Regulation, Mice, Mice, Inbred C57BL, Myocytes, Cardiac/metabolism, Oligonucleotide Array Sequence Analysis, Receptors, Glucocorticoid/genetics, Receptors, Glucocorticoid/metabolism, Receptors, Mineralocorticoid/genetics, Receptors, Mineralocorticoid/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 12:32
Last modification date
20/08/2019 13:16
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