beta-catenin-dependent and -independent effects of DeltaN-plakoglobin on epidermal growth and differentiation.

Details

Serval ID
serval:BIB_31666
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
beta-catenin-dependent and -independent effects of DeltaN-plakoglobin on epidermal growth and differentiation.
Journal
Molecular and Cellular Biology
Author(s)
Teulière J., Faraldo M.M., Shtutman M., Birchmeier W., Huelsken J., Thiery J.P., Glukhova M.A.
ISSN
0270-7306
Publication state
Published
Issued date
2004
Volume
24
Number
19
Pages
8649-8661
Language
english
Notes
Publication types: Journal Article
Abstract
Both beta-catenin and plakoglobin can stimulate the expression of Lef/Tcf target genes in vitro. beta-Catenin is known to associate with Lef/Tcf factors and to participate directly in transactivation in vivo, whereas the role of plakoglobin in transcriptional regulation has been less studied. To analyze the functions of plakoglobin in vivo, we generated transgenic mice expressing in the epidermis N-terminally truncated plakoglobin (DeltaN122-PG) lacking the glycogen synthase kinase 3beta phosphorylation sites and therefore protected against degradation (transgenic line K5-DeltaN122-PG). The expression of DeltaN122-PG led to the formation of additional hair germs, hyperplastic hair follicles, and noninvasive hair follicle tumors, a phenotype reminiscent of that induced by expression of N-terminally truncated beta-catenin. However, if expressed in beta-catenin-null epidermis, DeltaN122-PG did not induce new hair follicle germs and follicular tumors. Thus, DeltaN122-PG cannot substitute for beta-catenin in its signaling functions in vivo and the phenotype observed in K5-DeltaN122-PG mouse skin must be due to the aberrant activation of beta-catenin signaling. On the other hand, the expression of DeltaN122-PG in beta-catenin-null skin significantly increased the survival rate of mutant mice, rescued differentiation, and limited excessive proliferation in the interfollicular epidermis, suggesting that plakoglobin may be involved in the intracellular signaling events essential for epidermal differentiation.
Keywords
Animals, Cadherins/metabolism, Cell Differentiation/physiology, Cysts/metabolism, Cytoskeletal Proteins/genetics, Cytoskeletal Proteins/metabolism, Desmoplakins, Epidermis/growth &amp, development, Epidermis/physiology, Genes, Reporter, Mice, Mice, Transgenic, Proto-Oncogene Proteins c-myc/metabolism, Trans-Activators/metabolism, beta Catenin, gamma Catenin
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:30
Last modification date
20/08/2019 13:16
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