Connexin43 Inhibition Prevents Human Vein Grafts Intimal Hyperplasia.

Details

Serval ID
serval:BIB_31270505BF54
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Connexin43 Inhibition Prevents Human Vein Grafts Intimal Hyperplasia.
Journal
PloS one
Author(s)
Longchamp A., Allagnat F., Alonso F., Kuppler C., Dubuis C., Ozaki C.K., Mitchell J.R., Berceli S., Corpataux J.M., Déglise S., Haefliger J.A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
10
Number
9
Pages
e0138847
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Venous bypass grafts often fail following arterial implantation due to excessive smooth muscle cells (VSMC) proliferation and consequent intimal hyperplasia (IH). Intercellular communication mediated by Connexins (Cx) regulates differentiation, growth and proliferation in various cell types. Microarray analysis of vein grafts in a model of bilateral rabbit jugular vein graft revealed Cx43 as an early upregulated gene. Additional experiments conducted using an ex-vivo human saphenous veins perfusion system (EVPS) confirmed that Cx43 was rapidly increased in human veins subjected ex-vivo to arterial hemodynamics. Cx43 knock-down by RNA interference, or adenoviral-mediated overexpression, respectively inhibited or stimulated the proliferation of primary human VSMC in vitro. Furthermore, Cx blockade with carbenoxolone or the specific Cx43 inhibitory peptide 43gap26 prevented the burst in myointimal proliferation and IH formation in human saphenous veins. Our data demonstrated that Cx43 controls proliferation and the formation of IH after arterial engraftment.
Keywords
Animals, Cell Proliferation, Connexin 43/antagonists & inhibitors, Connexin 43/physiology, Humans, Hyperplasia/prevention & control, Male, Muscle, Smooth, Vascular/pathology, Rabbits, Tunica Intima/pathology, Vascular Grafting, Veins/transplantation
Pubmed
Web of science
Open Access
Yes
Create date
07/03/2021 13:56
Last modification date
08/03/2021 7:26
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