Murine clusterin: molecular cloning and mRNA localization of a gene associated with epithelial differentiation processes during embryogenesis

Details

Serval ID
serval:BIB_30ACC2D7D98E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Murine clusterin: molecular cloning and mRNA localization of a gene associated with epithelial differentiation processes during embryogenesis
Journal
Journal of Cell Biology
Author(s)
French  L. E., Chonn  A., Ducrest  D., Baumann  B., Belin  D., Wohlwend  A., Kiss  J. Z., Sappino  A. P., Tschopp  J., Schifferli  J. A.
ISSN
0021-9525 (Print)
Publication state
Published
Issued date
09/1993
Volume
122
Number
5
Pages
1119-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
Clusterin is a broadly distributed glycoprotein constitutively expressed by various tissues and cell types, that has been shown to be involved in cell-cell adhesion and expressed during cellular differentiation in vitro. To assess the suggested participation of clusterin in these processes in vivo, we have cloned the cDNA encoding murine clusterin and studied the cellular distribution of clusterin mRNA during murine embryogenesis. Sequence analysis of the cDNA encoding murine clusterin revealed 92 and 75% sequence identity with the rat and human cDNAs, respectively, and conservation of the predicted structural features which include alpha-helical regions and heparin-binding domains. From 12.5 d of development onwards, the clusterin gene is widely expressed in developing epithelia, and selectively localized within the differentiating cell layers of tissues such as the developing skin, tooth, and duodenum where proliferating and differentiating compartments are readily distinguished. In addition, transient and localized clusterin gene expression was detected in certain morphogenetically active epithelia. In the lung, abundant gene transcripts were detected in cuboidal epithelial cells of the terminal lung buds during branching morphogenesis, and in the kidney, clusterin gene expression in the epithelial cells of comma and S-shaped bodies coincided with the process of polarization. Our results demonstrate the in vivo expression of the clusterin gene by differentiating epithelial cells during murine embryogenesis, and provide novel evidence suggesting that clusterin may be involved in the differentiation and morphogenesis of certain epithelia.
Keywords
Amino Acid Sequence Animals Base Sequence Cell Adhesion Cell Differentiation/physiology Cloning, Molecular Clusterin DNA/analysis/genetics Decidua/chemistry/cytology/embryology Duodenum/chemistry/cytology/embryology Embryo/chemistry/cytology Embryo Implantation Embryonic and Fetal Development/*genetics/*physiology Epithelial Cells Epithelium/embryology Female Gene Expression Regulation Glycoproteins/*genetics In Situ Hybridization Kidney/chemistry/cytology/embryology Lung/cytology/*embryology Mice *Molecular Chaperones Molecular Sequence Data Morphogenesis/physiology RNA, Messenger/*analysis/*genetics Sequence Analysis, DNA Skin/chemistry/cytology/embryology Tooth/chemistry/cytology/embryology Transcription, Genetic Uterus/chemistry/cytology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:18
Last modification date
20/08/2019 13:15
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