Typing of Pneumocystis carinii f. sp. hominis by PCR-SSCP to indicate a high frequency of co-infections

Details

Serval ID
serval:BIB_309BA0372A2C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Typing of Pneumocystis carinii f. sp. hominis by PCR-SSCP to indicate a high frequency of co-infections
Journal
Journal of Medical Microbiology
Author(s)
Nahimana  A., Blanc  D. S., Francioli  P., Bille  J., Hauser  P. M.
ISSN
0022-2615
Publication state
Published
Issued date
08/2000
Peer-reviewed
Oui
Volume
49
Number
8
Pages
753-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
Broncho-alveolar lavage specimens from patients with Pneumocystis carinii pneumonia were investigated by PCR-single strand conformation polymorphism (SSCP) analysis of four genomic regions for P. carinii f. sp. hominis. In all, 32% of specimens produced two bands (one allele) for each of the four genomic regions, suggesting an infection with a single P.c. hominis type. The other specimens displayed more than two bands for at least one of the four genomic regions, suggesting several theoretical possibilities: co-infections, heterozygosity of diploid or polyploid organisms, or multicopy genes. Quantification of the different alleles and analysis of mixtures of specimens showed that different alleles of a genomic region were most often present in different proportions in a given specimen. In contrast, experiments with plasmid insertion of two alleles resulted in the detection of identical proportions of the two alleles. This suggests that neither heterozygosity of diploid organisms nor multicopy genes are responsible for the presence of two alleles. Unequal proportions are most likely explained by the occurrence of co-infections. The putative co-infecting types could be identified in the majority of specimens.
Keywords
Alleles Bronchoalveolar Lavage Fluid/microbiology DNA, Fungal/analysis Humans Pneumocystis/*classification/genetics Pneumonia, Pneumocystis/*microbiology *Polymerase Chain Reaction *Polymorphism, Single-Stranded Conformational
Pubmed
Web of science
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29/01/2008 16:20
Last modification date
20/08/2019 14:15
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