Plasma membrane cyclic nucleotide gated calcium channels control land plant thermal sensing and acquired thermotolerance.

Details

Serval ID
serval:BIB_3095C6BF2A1B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Plasma membrane cyclic nucleotide gated calcium channels control land plant thermal sensing and acquired thermotolerance.
Journal
Plant Cell
Author(s)
Finka A., Cuendet A.F., Maathuis F.J., Saidi Y., Goloubinoff P.
ISSN
1532-298X (Electronic)
ISSN-L
1040-4651
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
24
Number
8
Pages
3333-3348
Language
english
Abstract
Typically at dawn on a hot summer day, land plants need precise molecular thermometers to sense harmless increments in the ambient temperature to induce a timely heat shock response (HSR) and accumulate protective heat shock proteins in anticipation of harmful temperatures at mid-day. Here, we found that the cyclic nucleotide gated calcium channel (CNGC) CNGCb gene from Physcomitrella patens and its Arabidopsis thaliana ortholog CNGC2, encode a component of cyclic nucleotide gated Ca(2+) channels that act as the primary thermosensors of land plant cells. Disruption of CNGCb or CNGC2 produced a hyper-thermosensitive phenotype, giving rise to an HSR and acquired thermotolerance at significantly milder heat-priming treatments than in wild-type plants. In an aequorin-expressing moss, CNGCb loss-of-function caused a hyper-thermoresponsive Ca(2+) influx and altered Ca(2+) signaling. Patch clamp recordings on moss protoplasts showed the presence of three distinct thermoresponsive Ca(2+) channels in wild-type cells. Deletion of CNGCb led to a total absence of one and increased the open probability of the remaining two thermoresponsive Ca(2+) channels. Thus, CNGC2 and CNGCb are expected to form heteromeric Ca(2+) channels with other related CNGCs. These channels in the plasma membrane respond to increments in the ambient temperature by triggering an optimal HSR, leading to the onset of plant acquired thermotolerance.
Keywords
Adaptation, Biological, Amino Acid Sequence, Arabidopsis/genetics, Arabidopsis/growth & development, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Bryopsida/genetics, Bryopsida/growth & development, Calcium/metabolism, Cell Membrane/genetics, Cell Membrane/metabolism, Computational Biology, Cyclic Nucleotide-Gated Cation Channels/genetics, Cyclic Nucleotide-Gated Cation Channels/metabolism, Cytoplasm/genetics, Cytoplasm/metabolism, Gene Deletion, Gene Expression Regulation, Plant, Genes, Plant, Heat-Shock Response, Hot Temperature, Molecular Sequence Data, Phenotype, Phylogeny, Plants, Genetically Modified/genetics, Plants, Genetically Modified/growth & development, Signal Transduction, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
06/09/2012 14:21
Last modification date
20/08/2019 14:15
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