Oligoclonal expansions in the CD8(+)CD28(-) T cells largely explain the shorter telomeres detected in this subset: analysis by flow FISH.

Details

Serval ID
serval:BIB_30025B36D122
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Oligoclonal expansions in the CD8(+)CD28(-) T cells largely explain the shorter telomeres detected in this subset: analysis by flow FISH.
Journal
Human Immunology
Author(s)
Batliwalla F.M., Rufer N., Lansdorp P.M., Gregersen P.K.
ISSN
0198-8859
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
61
Number
10
Pages
951-958
Language
english
Abstract
We have previously reported that CD8(+)CD28(-) T cells have relatively shorter telomeres compared with CD8(+)CD28(+) T cells. Oligoclonal expansion is a common feature of CD8(+) T cells in human peripheral blood, and these expansions predominantly occur in the CD57(+)/CD28(-) population. We studied the telomere length in subsets of CD8(+) T cells using quantitative fluorescence in situ hybridization and flow cytometry (flow FISH). Our results confirm that CD8(+)CD28(-) T cells have shorter telomeres as compared with their CD28(+) counterpart cells. In addition, the oligoclonally expanded cells within the CD8(+)CD28(-) T cell subset generally have even shorter telomeres than the CD28(-) subset as a whole. We conclude that the presence of clonal expansions in the CD8(+)CD28(-) T cell population largely explain the shorter telomeres in this subset. These clonally expanded CD8(+)CD28(-) T cells generally have characteristics of terminally differentiated effector cells. Nevertheless, there is considerable individual variation in the degree of telomere shortening in these cells, which may reflect host genetic factors as well as the type and timing of the antigenic exposure.
Keywords
Antigens, CD28/analysis, CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, Clone Cells, Flow Cytometry/methods, Humans, In Situ Hybridization, Fluorescence/methods, Interferon-gamma/biosynthesis, Interleukin-2/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/analysis, T-Lymphocyte Subsets/cytology, T-Lymphocyte Subsets/immunology, Telomere/ultrastructure
Pubmed
Web of science
Create date
13/10/2009 9:16
Last modification date
20/08/2019 14:14
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