Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals.

Details

Serval ID
serval:BIB_2FFE761501C4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals.
Journal
Science advances
Author(s)
Torres-Méndez A., Pop S., Bonnal S., Almudi I., Avola A., Roberts RJV, Paolantoni C., Alcaina-Caro A., Martín-Anduaga A., Haussmann I.U., Morin V., Casares F., Soller M., Kadener S., Roignant J.Y., Prieto-Godino L., Irimia M.
ISSN
2375-2548 (Electronic)
ISSN-L
2375-2548
Publication state
Published
Issued date
28/01/2022
Peer-reviewed
Oui
Volume
8
Number
4
Pages
eabk0445
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Alternative splicing increases neuronal transcriptomic complexity throughout animal phylogeny. To delve into the mechanisms controlling the assembly and evolution of this regulatory layer, we characterized the neuronal microexon program in Drosophila and compared it with that of mammals. In nonvertebrate bilaterians, this splicing program is restricted to neurons by the posttranscriptional processing of the enhancer of microexons (eMIC) domain in Srrm234. In Drosophila, this processing is dependent on regulation by Elav/Fne. eMIC deficiency or misexpression leads to widespread neurological alterations largely emerging from impaired neuronal activity, as revealed by a combination of neuronal imaging experiments and cell type-specific rescues. These defects are associated with the genome-wide skipping of short neural exons, which are strongly enriched in ion channels. We found no overlap of eMIC-regulated exons between flies and mice, illustrating how ancient posttranscriptional programs can evolve independently in different phyla to affect distinct cellular modules while maintaining cell-type specificity.
Keywords
Alternative Splicing, Animals, Drosophila/genetics, Drosophila/metabolism, Drosophila Proteins/genetics, Mammals/genetics, Mammals/metabolism, Mice, Nerve Tissue Proteins/genetics, Neurons/metabolism, RNA Splicing, RNA-Binding Proteins
Pubmed
Web of science
Open Access
Yes
Create date
31/01/2022 10:44
Last modification date
11/06/2022 6:35
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