Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones
Details
Serval ID
serval:BIB_2FE7E51F2587
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones
Journal
Cell
ISSN
0092-8674 (Print)
Publication state
Published
Issued date
02/1981
Volume
23
Number
2
Pages
335-45
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
We have cloned circular unintegrated mouse mammary tumor virus (MMTV) DNA from infected rat hepatoma cells in bacteriophage lambda. Seven independent clones containing MMTV DNA of homogeneous length of 9 kb (five) or 10 kb (two) were identified. The five 9 kb clones had identical restriction maps consistent with that of 9 kb unintegrated DNA; the other two were aberrant. MMTV DNA inserts were purified, ligated and used for cotransfection of Ltk- cells together with a plasmid containing the thymidine kinase gene of herpes simplex virus. All Tk+ cell clones acquired new MMTV sequences and those transfected with the 9 kb MMTV DNA synthesized normal viral RNA and proteins. Viral gene expression was increased by the addition of dexamethasone.
Keywords
Animals Cell Line *Cloning, Molecular DNA Restriction Enzymes DNA, Viral/analysis Dexamethasone/*pharmacology Gene Expression Regulation/*drug effects Mammary Tumor Virus, Mouse/*genetics Mice RNA, Viral/analysis *Transfection Viral Proteins/biosynthesis
Pubmed
Web of science
Create date
25/01/2008 15:25
Last modification date
20/08/2019 14:14
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