Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury.

Details

Serval ID
serval:BIB_2FC1FA68898E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury.
Journal
Journal of Physiology
Author(s)
Poirot O., Berta T., Decosterd I., Kellenberger S.
ISSN
0022-3751[print], 0022-3751[linking]
Publication state
Published
Issued date
2006
Volume
576
Number
Pt 1
Pages
215-234
Language
english
Abstract
The H(+)-gated acid-sensing ion channels (ASICs) are expressed in dorsal root ganglion (DRG) neurones. Studies with ASIC knockout mice indicated either a pro-nociceptive or a modulatory role of ASICs in pain sensation. We have investigated in freshly isolated rat DRG neurones whether neurones with different ASIC current properties exist, which may explain distinct cellular roles, and we have investigated ASIC regulation in an experimental model of neuropathic pain. Small-diameter DRG neurones expressed three different ASIC current types which were all preferentially expressed in putative nociceptors. Type 1 currents were mediated by ASIC1a homomultimers and characterized by steep pH dependence of current activation in the pH range 6.8-6.0. Type 3 currents were activated in a similar pH range as type 1, while type 2 currents were activated at pH < 6. When activated by acidification to pH 6.8 or 6.5, the probability of inducing action potentials correlated with the ASIC current density. Nerve injury induced differential regulation of ASIC subunit expression and selective changes in ASIC function in DRG neurones, suggesting a complex reorganization of ASICs during the development of neuropathic pain. In summary, we describe a basis for distinct cellular functions of different ASIC types in small-diameter DRG neurones.
Keywords
Action Potentials/physiology, Animals, Calcium/pharmacokinetics, Cell Membrane Permeability/physiology, Cells, Cultured, Electrophysiology, Ganglia, Spinal/injuries, Ganglia, Spinal/metabolism, Gene Expression Regulation/physiology, Hydrogen-Ion Concentration, Male, Membrane Proteins/drug effects, Membrane Proteins/genetics, Nerve Tissue Proteins/drug effects, Nerve Tissue Proteins/genetics, Neuralgia/physiopathology, Nociceptors/metabolism, Rats, Rats, Sprague-Dawley, Rats, Wistar, Sodium Channels/drug effects, Sodium Channels/genetics, Spider Venoms/pharmacology, TRPV Cation Channels/genetics, TRPV Cation Channels/metabolism, Zinc/pharmacology
Pubmed
Web of science
Create date
24/01/2008 12:45
Last modification date
20/08/2019 13:14
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