Cytotoxic effect of lapatinib is restricted to human papillomavirus-positive head and neck squamous cell carcinoma cell lines.

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Ressource 1Download: ott-8-335.pdf (511.34 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_2F940D4E3636
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cytotoxic effect of lapatinib is restricted to human papillomavirus-positive head and neck squamous cell carcinoma cell lines.
Journal
OncoTargets and therapy
Author(s)
Fumagalli I., Dugue D., Bibault J.E., Clémenson C., Vozenin M.C., Mondini M., Deutsch E.
ISSN-L
1178-6930
Publication state
Published
Issued date
2015
Volume
8
Pages
335-345
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Lapatinib is a dual epidermal growth factor receptor (EGFR) and HER2 inhibitor. Overexpression of these receptors is frequently observed in head and neck squamous cell carcinoma (HNSCC). As growing proportion of HNSCC is characterized by human papillomavirus (HPV) infection, we aimed at evaluating the efficacy of lapatinib as function of HPV status in HNSCC cell lines.
Two HPV-positive and two HPV-negative HNSCC cell lines were used. Proliferation, cell cycle, and Annexin V assays were performed to test their sensitivity to lapatinib. Combination of lapatinib and ionizing radiation was evaluated with clonogenic survival assays. Akt, EGFR and HER2, and E6/E7 expression and activation were analyzed by immunoblotting and quantitative reverse transcription polymerase chain reaction.
Lapatinib reduced E6 and E7 expression and Akt phosphorylation, inhibited cell proliferation and induced cell death in HPV-positive cell lines. An additive effect of lapatinib with radiation was observed in these cells. Lapatinib had no effect on HPV-negative cells.
Lapatinib efficacy restricted to the HPV-positive cells suggests that HPV status could be a potential marker for enhanced response to lapatinib in HNSCC.

Pubmed
Open Access
Yes
Create date
14/02/2017 11:29
Last modification date
20/08/2019 13:14
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