S-nitrosoglutathione in rat cerebellum: identification and quantification by liquid chromatography-mass spectrometry.

Details

Serval ID
serval:BIB_2F191317349D
Type
Article: article from journal or magazin.
Collection
Publications
Title
S-nitrosoglutathione in rat cerebellum: identification and quantification by liquid chromatography-mass spectrometry.
Journal
Journal of Neurochemistry
Author(s)
Kluge I., Gutteck-Amsler U., Zollinger M., Do K.Q.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Publication state
Published
Issued date
1997
Peer-reviewed
Oui
Volume
69
Number
6
Pages
2599-2607
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Given the extreme lability and the facile inactivation of the messenger nitric oxide (NO) by many reactive biochemical species, it has been suggested that some intermediate compounds, for example, S-nitrosothiols, may act to stabilize NO and at the same time to preserve its biological activity. To test this hypothesis, we investigated if the S-nitrosothiol of glutathione, which is the predominant low molecular weight thiol in CNS, is present in the rat brain. The HPLC analysis of cerebellar extract from [35S]cysteine-prelabeled slices suggested that S-nitrosoglutathione (GSNO) was indeed present in rat brain. To detect endogenous GSNO, a methodology based on liquid chromatography-mass spectrometry was developed. Besides an unequivocal identification of the endogenous GSNO, this method also permitted its precise quantification using 15N-labeled GSNO ([15N]GSNO) as internal standard. GSNO level in adult cerebellum amounts to 15.4 +/- 1.4 pmol/mg of protein. This is the first direct demonstration of the presence of endogenous GSNO in CNS. The packaging of NO in the form of GSNO might serve to facilitate its transport, prolong its life, and target its delivery to specific effectors.
Keywords
Animals, Cerebellum/metabolism, Chromatography, High Pressure Liquid, Glutathione/analogs & derivatives, Glutathione/metabolism, Nitroso Compounds/metabolism, Platelet Aggregation Inhibitors/metabolism, Rats, Rats, Inbred Strains, S-Nitrosoglutathione, Spectrometry, Mass, Fast Atom Bombardment
Pubmed
Web of science
Create date
07/03/2014 9:37
Last modification date
20/08/2019 13:13
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