Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata.

Details

Serval ID
serval:BIB_2E964B9A5040
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata.
Journal
NMR in Biomedicine
Author(s)
Xin Lijing, Gambarota Giulio, Duarte Joao M. N., Mlynarik Vladimir, Gruetter Rolf
ISSN
1099-1492[electronic], 0952-3480[linking]
Publication state
Published
Issued date
2010
Volume
23
Number
9
Pages
1097-1102
Language
english
Abstract
The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine. The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE?=?2.8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat. Glycine was quantified in MO at TE?=?2.8 ms with a Cramér-Rao lower bound (CRLB) of approximately 5%. As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE?=?20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance. The other 14 metabolites composing the neurochemical profile were quantified in vivo in MO with CRLBs below 25%. Absolute concentrations of metabolites in MO, such as glutamate, glutamine, ?-aminobutyrate, taurine and glycine, were in the range of previous in vitro quantifications in tissue extracts. Compared with the other regions, MO had a three-fold higher glycine concentration, and was characterised by reduced (p?<?0.001) concentrations of glutamate (-50?±?4%), glutamine (-54?±?3%) and taurine (-78?±?3%). This study suggests that the functional specialisation of distinct brain regions is reflected in the neurochemical profile.
Keywords
Glycine, Neurochemical Profile, Medulla Oblongata, NMR Spectroscopy, Short-Echo-Time, H-1-NMR Spectroscopy, Spinal-Cord, Human Brain, Regional Changes, Quantification, Metabolites, Receptors, Subunit, Spectra
Pubmed
Web of science
Create date
09/12/2010 9:23
Last modification date
20/08/2019 13:13
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