Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata.
Details
Serval ID
serval:BIB_2E964B9A5040
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata.
Journal
NMR in Biomedicine
ISSN
1099-1492[electronic], 0952-3480[linking]
Publication state
Published
Issued date
2010
Volume
23
Number
9
Pages
1097-1102
Language
english
Abstract
The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine. The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE?=?2.8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat. Glycine was quantified in MO at TE?=?2.8 ms with a Cramér-Rao lower bound (CRLB) of approximately 5%. As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE?=?20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance. The other 14 metabolites composing the neurochemical profile were quantified in vivo in MO with CRLBs below 25%. Absolute concentrations of metabolites in MO, such as glutamate, glutamine, ?-aminobutyrate, taurine and glycine, were in the range of previous in vitro quantifications in tissue extracts. Compared with the other regions, MO had a three-fold higher glycine concentration, and was characterised by reduced (p?<?0.001) concentrations of glutamate (-50?±?4%), glutamine (-54?±?3%) and taurine (-78?±?3%). This study suggests that the functional specialisation of distinct brain regions is reflected in the neurochemical profile.
Keywords
Glycine, Neurochemical Profile, Medulla Oblongata, NMR Spectroscopy, Short-Echo-Time, H-1-NMR Spectroscopy, Spinal-Cord, Human Brain, Regional Changes, Quantification, Metabolites, Receptors, Subunit, Spectra
Pubmed
Web of science
Create date
09/12/2010 10:23
Last modification date
20/08/2019 14:13