Integration of replication-defective R68.45-like plasmids into the Pseudomonas aeruginosa chromosome.

Details

Serval ID
serval:BIB_2E87B9BC7C91
Type
Article: article from journal or magazin.
Collection
Publications
Title
Integration of replication-defective R68.45-like plasmids into the Pseudomonas aeruginosa chromosome.
Journal
Journal of General Microbiology
Author(s)
Reimmann C., Rella M., Haas D.
ISSN
0022-1287 (Print)
ISSN-L
0022-1287
Publication state
Published
Issued date
1988
Volume
134
Number
6
Pages
1515-1523
Language
english
Notes
l'issn correspond au titre
Abstract
R68.45 and other similar broad-host-range (IncP) plasmids carrying a tandem repeat of the 2.1 kb insertion element IS21 mobilize the chromosome of many different Gram-negative bacteria. To analyse the structure of R68.45-chromosome cointegrates, whose involvement in the mobilization process had been postulated previously, we selected for the stable integration of R68.45-like plasmids into the Pseudomonas aeruginosa chromosome. Two plasmids were chosen: pME28, a transfer-deficient, mobilizable RP1 derivative with an inactive replication control (trfA) gene, and pME487, an R68.45 derivative with a trfA(ts) mutation causing temperature-sensitive replication. Chromosomally integrated pME28 and pME487 were found to be flanked by single IS21 elements. This structure is in agreement with a 'cut-and-paste' mode of R68.45 transposition. pME28 and pME487 showed a low specificity of insertion but rarely (less than 0.1%) induced auxotrophic mutations. Hfr (high-frequency-of-recombination) donors of P. aeruginosa could be obtained by chromosomal integration of pME487 or pME28; in the latter case, the transfer functions lacking from pME28 had to be provided in trans on an autonomous plasmid. Hfr donors gave higher conjugational linkage and transferred longer stretches of the P. aeruginosa chromosome than did R68.45 donors. This suggests that the integration of R68.45 into the donor chromosome is short-lived in P. aeruginosa.
Keywords
Chromosomes, Bacterial, Conjugation, Genetic, DNA Replication, DNA, Bacterial/genetics, Escherichia coli, Mutation, Nucleic Acid Hybridization, Pseudomonas aeruginosa/genetics, R Factors
Pubmed
Web of science
Create date
24/01/2008 14:00
Last modification date
20/08/2019 13:13
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