Antagonistic peptide technology for functional dissection of CLE peptides revisited.

Details

Ressource 1Download: serval:BIB_2E422503DFEF.P001 (5506.73 [Ko])
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Serval ID
serval:BIB_2E422503DFEF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antagonistic peptide technology for functional dissection of CLE peptides revisited.
Journal
Journal of Experimental Botany
Author(s)
Czyzewicz N., Wildhagen M., Cattaneo P., Stahl Y., Pinto K.G., Aalen R.B., Butenko M.A., Simon R., Hardtke C.S., De Smet I.
ISSN
1460-2431 (Electronic)
ISSN-L
0022-0957
Publication state
Published
Issued date
2015
Volume
66
Number
17
Pages
5367-5374
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide approaches (in the broad sense) can be very useful, but these approaches do not work in all cases and require a deep insight on the interaction between the ligand and its receptor to be successful. This, as well as peptide ligand structure considerations, should be taken into account before ordering a wide range of synthetic peptide variants and/or generating transgenic plants.
Keywords
Amino Acid Sequence, Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/chemistry, Arabidopsis Proteins/genetics, Gene Expression Regulation, Plant, Magnetic Resonance Spectroscopy/methods, Peptides/genetics, Peptides/metabolism, Sequence Alignment
Pubmed
Web of science
Open Access
Yes
Create date
04/09/2015 8:16
Last modification date
25/09/2019 6:08
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