A simple method for cardiac surgery in rats

Details

Serval ID
serval:BIB_2E38FB859F07
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A simple method for cardiac surgery in rats
Journal
Cell Transplantation
Author(s)
Connold  A. L., Frischknecht  R., Vrbova  G.
ISSN
0963-6897 (Print)
Publication state
Published
Issued date
06/1996
Peer-reviewed
Oui
Volume
5
Number
3
Pages
405-9
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: May-Jun
Abstract
In our laboratory we have developed a relatively simple method for cardiac surgery in rats. The operation is carried out through a small incision in the chest wall using inexpensive equipment. This method allows for the delivery of tissue fragments and cells from a donor rat to an intact or damaged area of ventricular myocardium of a host rat, with easy subsequent localisation of the transplanted/grafted tissue. The rats recover well after the surgery and survive for long periods of time. The technique could also be used for the direct injection of chemicals or molecular probes into the heart. In our experiments we have found that embryonic rat cardiomyocytes that have been transplanted into adult host rat ventricular myocardium using this method survive and develop characteristics typical of heart muscle, thus indicating that using this technique the host heart offers a favourable environment for the transplanted embryonic heart cells.
Keywords
Animals Cardiac Surgical Procedures/*methods Cell Transplantation/*methods Cryopreservation/*methods Female Fetal Tissue Transplantation/*methods *Heart Heart Ventricles Intubation, Intratracheal/instrumentation/methods Myocardial Contraction Myocardium/*cytology Rats Rats, Inbred F344
Pubmed
Web of science
Create date
25/01/2008 8:34
Last modification date
20/08/2019 13:12
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