Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations

Details

Serval ID
serval:BIB_2E280C4A083E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
Journal
RSC Advances
Author(s)
Duchamp Margaux, Dahoun Thamani, Vaillier Clarisse, Arnaud Marion, Bobisse Sara, Coukos George, Harari Alexandre, Renaud Philippe
ISSN
2046-2069
Publication state
Published
Issued date
2019
Volume
9
Number
70
Pages
41066-41073
Language
english
Abstract
In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell-cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specific locations, then relied on hydrodynamic filtering principles, the flowing cells from the second cell population are placed in contact with the trapped ones, through a roll-over mechanism. The rolling cells interact with the serially trapped cells one after the other. The proposed microfluidic phenomenon was characterized with beads. We have shown the validity of our method by detecting the capacity of olfactory receptors to induce adhesion of cell doublets overexpressing these receptors. We report here the first controlled on-flow single cell resolution cell-cell interaction assay in a microfluidic device for future application in cell-cell interactions-based cell library screenings.
Keywords
General Chemistry, General Chemical Engineering
Web of science
Open Access
Yes
Create date
18/02/2020 9:20
Last modification date
26/08/2020 5:26
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