Inducible malondialdehyde pools in zones of cell proliferation and developing tissues in Arabidopsis.

Details

Serval ID
serval:BIB_2E0539DE0DAA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inducible malondialdehyde pools in zones of cell proliferation and developing tissues in Arabidopsis.
Journal
Journal of Biological Chemistry
Author(s)
Schmid-Siegert E., Loscos J., Farmer E.E.
ISSN
1083-351X (Electronic)
ISSN-L
0021-9258
Publication state
Published
Issued date
2012
Volume
287
Number
12
Pages
8954-8962
Language
english
Abstract
Malondialdehyde (MDA) is a natural and widespread genotoxin. Given its potentially deleterious effects, it is of interest to establish the identities of the cell types containing this aldehyde. We used in situ chemical trapping with 2-thiobarbituric acid and mass spectrometry with a deuterated standard to characterize MDA pools in the vegetative phase in Arabidopsis thaliana. In leaves, MDA occurred predominantly in the intracellular compartment of mesophyll cells and was enriched in chloroplasts where it was derived primarily from triunsaturated fatty acids (TFAs). High levels of MDA (most of which was unbound) were found within dividing cells in the root tip cell proliferation zone. The bulk of this MDA did not originate from TFAs. We confirmed the localization of MDA in transversal root sections. In addition to MDA in proliferating cells near the root tip we found evidence for the presence of MDA in pericyle cells. Remodeling of non-TFA-derived MDA pools occurred when seedlings were infected with the fungus Botrytis cinerea. Treatment of uninfected seedlings with mediators of plant stress responses (jasmonic acid or salicylic acid) increased seedling MDA levels over 20-fold. In summary, major pools of MDA are associated with cell division foci containing stem cells. The aldehyde is pathogen-inducible in these regions and its levels are increased by cellular mediators that impact defense and growth.
Keywords
Arabidopsis/cytology, Arabidopsis/growth & development, Botrytis/physiology, Cell Proliferation, Chloroplasts/metabolism, Fatty Acids/metabolism, Gene Expression Regulation, Plant, Malondialdehyde/metabolism, Mesophyll Cells/cytology, Mesophyll Cells/metabolism, Plant Diseases/microbiology, Plant Roots/cytology, Plant Roots/genetics
Pubmed
Web of science
Open Access
Yes
Create date
22/10/2012 10:42
Last modification date
20/08/2019 13:12
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