Quantifying Infra-slow Dynamics of Spectral Power and Heart Rate in Sleeping Mice.

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_2DED7862F8DB
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Quantifying Infra-slow Dynamics of Spectral Power and Heart Rate in Sleeping Mice.
Journal
Journal of visualized experiments
Author(s)
Fernandez LMJ, Lecci S., Cardis R., Vantomme G., Béard E., Lüthi A.
ISSN
1940-087X (Electronic)
ISSN-L
1940-087X
Publication state
Published
Issued date
02/08/2017
Peer-reviewed
Oui
Number
126
Pages
1-9
Language
english
Notes
Publication types: Journal Article ; Video-Audio Media
Publication Status: epublish
Abstract
Three vigilance states dominate mammalian life: wakefulness, non-rapid eye movement (non-REM) sleep, and REM sleep. As more neural correlates of behavior are identified in freely moving animals, this three-fold subdivision becomes too simplistic. During wakefulness, ensembles of global and local cortical activities, together with peripheral parameters such as pupillary diameter and sympathovagal balance, define various degrees of arousal. It remains unclear the extent to which sleep also forms a continuum of brain states-within which the degree of resilience to sensory stimuli and arousability, and perhaps other sleep functions, vary gradually-and how peripheral physiological states co-vary. Research advancing the methods to monitor multiple parameters during sleep, as well as attributing to constellations of these functional attributes, is central to refining our understanding of sleep as a multifunctional process during which many beneficial effects must be executed. Identifying novel parameters characterizing sleep states will open opportunities for novel diagnostic avenues in sleep disorders. We present a procedure to describe dynamic variations of mouse non-REM sleep states via the combined monitoring and analysis of electroencephalogram (EEG)/electrocorticogram (ECoG), electromyogram (EMG), and electrocardiogram (ECG) signals using standard polysomnographic recording techniques. Using this approach, we found that mouse non-REM sleep is organized into cycles of coordinated neural and cardiac oscillations that generate successive 25-s intervals of high and low fragility to external stimuli. Therefore, central and autonomic nervous systems are coordinated to form behaviorally distinct sleep states during consolidated non-REM sleep. We present surgical manipulations for polysomnographic (i.e., EEG/EMG combined with ECG) monitoring to track these cycles in the freely sleeping mouse, the analysis to quantify their dynamics, and the acoustic stimulation protocols to assess their role in the likelihood of waking up. Our approach has already been extended to human sleep and promises to unravel common organizing principles of non-REM sleep states in mammals.

Keywords
Animals, Brain/physiology, Brain/surgery, Electrocorticography/methods, Electroencephalography/methods, Electromyography/methods, Heart Rate/physiology, Humans, Mice, Inbred C57BL, Polysomnography/methods, Sleep/physiology, Sleep, REM/physiology, Wakefulness/physiology
Pubmed
Web of science
Create date
06/09/2017 10:30
Last modification date
20/08/2019 13:12
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