Adherence of different Candida dubliniensis isolates in the presence of fluconazole
Details
Serval ID
serval:BIB_2D5D07D2D814
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Adherence of different Candida dubliniensis isolates in the presence of fluconazole
Journal
AIDS
ISSN
0269-9370 (Print)
Publication state
Published
Issued date
06/2002
Volume
16
Number
9
Pages
1237-44
Notes
Comparative Study
Journal Article --- Old month value: Jun 14
Journal Article --- Old month value: Jun 14
Abstract
BACKGROUND: The recently described yeast species Candida dubliniensis is closely related to C. albicans and has been recovered predominantly from the oral cavities of HIV-infected individuals and AIDS patients who are often receiving fluconazole as prophylactic or therapeutic treatment for oropharyngeal candidiasis. Like C. albicans, C. dubliniensis secretes aspartic proteinases which in C. albicans have been shown to be involved in adherence. OBJECTIVE: To explain the increasing prevalence of C. dubliniensis in AIDS patients and to investigate the virulence factors of this yeast. METHODS: An in vitro assay was developed to compare the adherence to epithelial cells of C. dubliniensis from HIV-patients with that of C. albicans. RESULTS: All C. albicans isolates adhered better than the 22 C. dubliniensis isolates. In the presence of fluconazole, the C. dubliniensis isolates tested showed increased adherence as compared with controls without fluconazole. In contrast, all C. albicans isolates decreased in adherence to epithelial cells in the presence of fluconazole independently of their in vitro susceptibility to this drug. Proteinase antigens are present on the surface of C. dubliniensis cells adherent to epithelial target cells. In the presence of fluconazole this proteinase antigen was more strongly expressed. CONCLUSION: Increased adherence of C. dubliniensis strains in the presence of fluconazole could explain its high recovery rate from HIV-positive patients in recent years. The induction of proteinase secretion in the presence of fluconazole found for most of the C. dubliniensis isolates could be one of the factors involved in adherence.
Keywords
AIDS-Related Opportunistic Infections/*microbiology
Candida/classification/*drug effects/metabolism/*pathogenicity
Candida albicans/cytology/drug effects/metabolism/pathogenicity
Candidiasis, Oral/*microbiology
Cell Adhesion
Endopeptidases/metabolism
Epithelial Cells/microbiology
Fluconazole/*pharmacology
Humans
Mouth Mucosa/microbiology
Pubmed
Web of science
Create date
25/01/2008 16:47
Last modification date
20/08/2019 13:12