Plasma angiotensins under sustained converting enzyme inhibition with enalapril in normal humans

Details

Serval ID
serval:BIB_2D3EF2795944
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Plasma angiotensins under sustained converting enzyme inhibition with enalapril in normal humans
Journal
Journal of Hypertension. Supplement
Author(s)
Nussberger  J., Brunner  D. B., Waeber  B., Brunner  H. R.
ISSN
0952-1178 (Print)
Publication state
Published
Issued date
12/1985
Volume
3
Number
3
Pages
S269-70
Notes
Journal Article --- Old month value: Dec
Abstract
During converting enzyme inhibition, a striking difference is found between plasma immunoreactive angiotensin (ANG) II and ANG-(1-8)octapeptide as specifically measured after high performance liquid chromatography (HPLC). The relative contributions to this difference of cross-reacting ANG I and shorter ANG peptides other than (1-8)octapeptide are not known. Eight normal volunteers were given a daily single oral dose of enalapril (20 mg) for 4 days. Plasma levels of ANG-(1-8)octapeptide, (2-8)heptapeptide, (3-8)hexapeptide and (4-8)pentapeptide as well as blood concentrations of ANG I and plasma angiotensin converting enzyme activity were measured before and 4 h post drug on the first and the last day of treatment. Plasma peptides were extracted with bonded-phase silica and separated by isocratic reversed-phase HPLC before radio-immunoassay. Significant cross-reactivity of an antiserum permitted the individual measurement of different ANG peptides and metabolites in plasma. During converting enzyme inhibition, the plasma levels of ANG metabolites tended to decrease, but they remained measurable. Thus, depending on the specificity of the antiserum used, conventional measurement of immunoreactive ANG II underestimates to various degrees the inhibition of angiotensin converting enzyme.
Keywords
Adult Angiotensin-Converting Enzyme Inhibitors/*pharmacology Angiotensins/*blood Chromatography, High Pressure Liquid Cross Reactions Enalapril/*pharmacology Humans Peptide Fragments/blood/immunology Peptidyl-Dipeptidase A/blood Radioimmunoassay
Pubmed
Web of science
Create date
05/03/2008 16:41
Last modification date
20/08/2019 13:12
Usage data