Combination of stromal cell-derived factor-1 and collagen-glycosaminoglycan scaffold delays contraction and accelerates reepithelialization of dermal wounds in wild-type mice.

Details

Serval ID
serval:BIB_2D33188BD6AA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Combination of stromal cell-derived factor-1 and collagen-glycosaminoglycan scaffold delays contraction and accelerates reepithelialization of dermal wounds in wild-type mice.
Journal
Wound repair and regeneration
Author(s)
Sarkar A., Tatlidede S., Scherer S.S., Orgill D.P., Berthiaume F.
ISSN
1524-475X (Electronic)
ISSN-L
1067-1927
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
19
Number
1
Pages
71-79
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
While dermal substitutes can mitigate scarring and wound contraction, a significant drawback of current dermal replacement technologies is the apparent delay in vascular ingrowth compared with conventional skin grafts. Herein, we examined the effect of the chemokine stromal cell-derived factor-1 (SDF-1) on the performance of a porous collagen-glycosaminoglycan dermal analog in excisional wounds in mice. C57BL/6 mice with 1 cm × 1 cm dorsal full-thickness wounds were covered with a collagen-glycosaminoglycan scaffold, followed by four daily topical applications of 1 μg SDF-1 or phosphate-buffered saline vehicle. Some animals were also pretreated with five daily doses of 300 mg/kg granulocyte colony-stimulating factor. Animals treated with SDF-1 and no granulocyte colony-stimulating factor reepithelialized 36% faster than vehicle controls (16 vs. 25 days), and exhibited less wound contraction on postwounding day 18 (∼ 35% greater wound area) plus three-fold longer neoepidermis formed than controls. Conversely, granulocyte colony-stimulating factor promoted contraction and no epidermal regeneration. Early (postwounding Day 3) inflammatory cell infiltration in the SDF-1-treated group was 86% less, while the fraction of proliferating cells (positive Ki67 staining) was 32% more, when compared with controls. These results suggest that SDF-1 simultaneously delays contraction and promotes reepithelialization and may improve the wound-healing performance of skin substitutes.

Keywords
Animals, Chemokine CXCL12/therapeutic use, Collagen, Dermis/injuries, Glycosaminoglycans, Granulocyte Colony-Stimulating Factor/therapeutic use, Male, Mice, Mice, Inbred C57BL, Skin, Artificial, Tissue Scaffolds, Wound Healing/physiology, Wounds, Penetrating/pathology, Wounds, Penetrating/therapy
Pubmed
Web of science
Create date
16/01/2018 21:49
Last modification date
20/08/2019 13:12
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