Preparative isolation of closely-related xanthones from Gentianella amarella ssp. acuta by High-speed countercurrent chromatography.

Details

Serval ID
serval:BIB_2CDAC313183C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Preparative isolation of closely-related xanthones from Gentianella amarella ssp. acuta by High-speed countercurrent chromatography.
Journal
Phytochemical analysis
Author(s)
Urbain A., Marston A., Batsuren D., Purev O., Hostettmann K.
ISSN
1099-1565 (Electronic)
ISSN-L
0958-0344
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
19
Number
6
Pages
514-519
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A methanolic extract from Gentianella amarella ssp. acuta was shown to contain several xanthones exhibiting acetylcholinesterase inhibitory activity. These xanthones were difficult to separate by conventional LC techniques, which prevented the isolation of pure compounds in sufficient amounts to perform in-depth biological testing.
To develop a suitable preparative method for the separation of closely related xanthones.
The methanolic extract was first partitioned with solvents of increasing polarity, in order to separate glycosides from xanthone aglycones. High-speed countercurrent chromatography (HSCCC) methods were then optimised for the fractionation of both polar and non-polar extracts.
The use of HSCCC enabled the separation of xanthones which co-eluted by HPLC. Ten closely related xanthones--three of which were isomeric--were successfully isolated by developing suitable solvent systems. All compounds were obtained in sufficient amounts to allow further biological assays (e.g. up to 250 mg), including even minor compounds that were not detectable by analytical HPLC.
The orthogonality of HSCCC with HPLC and the absence of solid-phase supports enabled the detection, separation and preparative isolation of closely related compounds which were difficult to resolve by other techniques.
Keywords
Chromatography, High Pressure Liquid/methods, Countercurrent Distribution/methods, Gentianella/chemistry, Magnetic Resonance Spectroscopy, Mass Spectrometry, Solvents/chemistry, Spectrophotometry, Ultraviolet, Xanthones/isolation & purification
Pubmed
Web of science
Create date
29/01/2009 22:13
Last modification date
15/01/2024 11:23
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