Regulated laminin-332 expression in human islets of Langerhans

Details

Serval ID
serval:BIB_2C87E7D766DB
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regulated laminin-332 expression in human islets of Langerhans
Journal
Faseb Journal
Author(s)
Armanet M., Wojtusciszyn A., Morel P., Parnaud G., Rousselle P., Sinigaglia C., Berney T., Bosco D.
ISSN
0892-6638
Publication state
Published
Issued date
12/2009
Volume
23
Number
12
Pages
4046-4055
Language
english
Notes
525dl
Times Cited:13
Cited References Count:64
Abstract
Laminin-332 (LN-332) is a basement membrane component known to exert a beneficial effect on rat pancreatic beta cells in vitro. In this work, we analyzed the expression of LN-332 in human islets, its expression after inflammatory insults by cytokines, and the molecular mechanisms responsible for this effect. By Western blotting and RT-PCR, we showed that LN-332 was expressed in isolated human islets. By immunofluorescence on pancreas sections, we observed that labeling was confined to endocrine cells in islets. Confocal microscopy analysis on isolated islet cells revealed that labeling was granular but did not colocalize with hormone secretory granules. LN-332 was most abundant in cultured islets compared to freshly isolated islets and was found in culture medium, which suggests that it was secreted by islets. When islets were exposed to interleukin (IL)-1 beta, expression and secretion of LN-332 increased as compared to control. No effect was observed with tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma. LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3-K) activity, inhibited culture- and IL-1 beta-induced LN-332 expression in islets. These results show that LN-332, known to have some beneficial effect on beta cells in vitro, is produced and secreted by endocrine islet cells and is up-regulated by stressing conditions such as culture and IL-1 beta-exposure.-Armanet, M., Wojtusciszyn, A., Morel, P., Parnaud, G., Rousselle, P., Sinigaglia, C., Berney, T., Bosco, D. Regulated laminin-332 expression in human islets of Langerhans. FASEB J. 23, 4046-4055 (2009). www.fasebj.org
Keywords
extracellular matrix, cytokines, pi3-k, nf-kappa-b, basement-membrane, in-vitro, phosphatidylinositol 3-kinase, extracellular-matrix, cell-proliferation, pancreatic-islets, gamma-2 chain, beta-cells, interleukin-1
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14/06/2021 8:58
Last modification date
18/09/2021 5:38
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