Basal and stimulated lactate fluxes in primary cultures of astrocytes are differentially controlled by distinct proteins.

Details

Serval ID
serval:BIB_2C3759B420E6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Basal and stimulated lactate fluxes in primary cultures of astrocytes are differentially controlled by distinct proteins.
Journal
Journal of Neurochemistry
Author(s)
Maekawa F., Minehira K., Kadomatsu K., Pellerin L.
ISSN
1471-4159
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
107
Number
3
Pages
789-798
Language
english
Abstract
Lactate release by astrocytes is postulated to be of importance for neuroenergetics but its regulation is poorly understood. Basigin, a chaperone protein for specific monocarboxylate transporters (MCTs), represents a putatively important regulatory element for lactate fluxes. Indeed, basigin knockdown by RNA interference in primary cultures of astrocytes partially reduced both proton-driven lactate influx and efflux. But more strikingly, enhancement of lactate efflux induced by glutamate was prevented while the effect of sodium azide was significantly reduced by treatment of cultured astrocytes with anti-basigin small interfering RNA. Enhancement of glucose utilization was unaffected under the same conditions. Basal lactate uptake and release were significantly reduced by MCT1 knockdown, even more so than with basigin knockdown, whereas glutamate-driven or sodium azide-induced enhancement of lactate release was not inhibited by either MCT1, 2, or 4 small interfering RNAs. In conclusion, MCT1 plays a pivotal role in the control of basal proton-driven lactate flux in astrocytes while basigin is only partly involved, most likely via its interaction with MCT1. In contrast, basigin appears to critically regulate the enhancement of lactate release caused by glutamate (or sodium azide) but via an effect on another unidentified transporter at least present in astrocytes in vitro.
Keywords
Animals, Antigens, CD147, Astrocytes, Cells, Cultured, Enzyme Inhibitors, Glutamic Acid, Lactates, Mice, Monocarboxylic Acid Transporters, RNA, Small Interfering, Sodium Azide, Symporters, Transfection
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2009 22:13
Last modification date
20/08/2019 13:11
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