Live cell dynamics of production, explosive release and killing activity of phage tail-like weapons for Pseudomonas kin exclusion.

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Ressource 1Download: Vacheron_et_al-2021-Communications_Biology.pdf (3925.65 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_2C21215297A5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Live cell dynamics of production, explosive release and killing activity of phage tail-like weapons for Pseudomonas kin exclusion.
Journal
Communications biology
Author(s)
Vacheron J., Heiman C.M., Keel C.
ISSN
2399-3642 (Electronic)
ISSN-L
2399-3642
Publication state
Published
Issued date
19/01/2021
Peer-reviewed
Oui
Volume
4
Number
1
Pages
87
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Interference competition among bacteria requires a highly specialized, narrow-spectrum weaponry when targeting closely-related competitors while sparing individuals from the same clonal population. Here we investigated mechanisms by which environmentally important Pseudomonas bacteria with plant-beneficial activity perform kin interference competition. We show that killing between phylogenetically closely-related strains involves contractile phage tail-like devices called R-tailocins that puncture target cell membranes. Using live-cell imaging, we evidence that R-tailocins are produced at the cell center, transported to the cell poles and ejected by explosive cell lysis. This enables their dispersal over several tens of micrometers to reach targeted cells. We visualize R-tailocin-mediated competition dynamics between closely-related Pseudomonas strains at the single-cell level, both in non-induced condition and upon artificial induction. We document the fatal impact of cellular self-sacrifice coupled to deployment of phage tail-like weaponry in the microenvironment of kin bacterial competitors, emphasizing the necessity for microscale assessment of microbial competitions.
Pubmed
Web of science
Open Access
Yes
Create date
20/01/2021 18:39
Last modification date
25/02/2021 7:26
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