The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila.

Details

Serval ID
serval:BIB_2C08A82FBFB0
Type
Article: article from journal or magazin.
Collection
Publications
Title
The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila.
Journal
Development
Author(s)
Roignant J.Y., Legent K., Janody F., Treisman J.E.
ISSN
1477-9129 (Electronic)
ISSN-L
0950-1991
Publication state
Published
Issued date
01/2010
Peer-reviewed
Oui
Volume
137
Number
2
Pages
273-281
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Abstract
Development involves the establishment of boundaries between fields specified to differentiate into distinct tissues. The Drosophila larval eye-antennal imaginal disc must be subdivided into regions that differentiate into the adult eye, antenna and head cuticle. We have found that the transcriptional co-factor Chip is required for cells at the ventral eye-antennal disc border to take on a head cuticle fate; clones of Chip mutant cells in this region instead form outgrowths that differentiate into ectopic eye tissue. Chip acts independently of the transcription factor Homothorax, which was previously shown to promote head cuticle development in the same region. Chip and its vertebrate CLIM homologues have been shown to form complexes with LIM-homeodomain transcription factors, and the domain of Chip that mediates these interactions is required for its ability to suppress the eye fate. We show that two LIM-homeodomain proteins, Arrowhead and Lim1, are expressed in the region of the eye-antennal disc affected in Chip mutants, and that both require Chip for their ability to suppress photoreceptor differentiation when misexpressed in the eye field. Loss-of-function studies support the model that Arrowhead and Lim1 act redundantly, using Chip as a co-factor, to prevent retinal differentiation in regions of the eye disc destined to become ventral head tissue.
Keywords
Animals, Cell Differentiation/genetics, Cell Differentiation/physiology, Drosophila/embryology, Drosophila/genetics, Drosophila/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila Proteins/physiology, Eye/embryology, Gene Expression Regulation, Developmental/genetics, Gene Expression Regulation, Developmental/physiology, Homeodomain Proteins/genetics, Homeodomain Proteins/metabolism, Homeodomain Proteins/physiology, Immunohistochemistry, In Situ Hybridization, LIM-Homeodomain Proteins, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Nuclear Proteins/physiology, Photoreceptor Cells/cytology, Photoreceptor Cells/metabolism, Transcription Factors/genetics, Transcription Factors/physiology
Pubmed
Web of science
Open Access
Yes
Create date
28/10/2019 12:59
Last modification date
29/10/2019 6:26
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