Public health guidelines recommend exercise as a key lifestyle intervention for promoting and maintaining healthy sleep function and reducing disease risk. However, strenuous evening exercise may disrupt sleep due to heightened sympathetic arousal. This study examines the association between strenuous evening exercise and objective sleep, using data from 14,689 physically active individuals who wore a biometric device during a one-year study interval (4,084,354 person-nights). Here we show later exercise timing and higher exercise strain are associated with delayed sleep onset, shorter sleep duration, lower sleep quality, higher nocturnal resting heart rate, and lower nocturnal heart rate variability. Regardless of strain, exercise bouts ending ≥4 hours before sleep onset are not associated with changes in sleep. Our results suggest evening exercise-particularly involving high exercise strain-may disrupt subsequent sleep and nocturnal autonomic function. Individuals aiming to improve sleep health may benefit from concluding exercise at least 4 hours before sleep onset or electing lighter strain exercises within this window.