Expression and developmental regulation of Ehk-1, a neuronal Elk-like receptor tyrosine kinase in brain.

Details

Serval ID
serval:BIB_2BF16CA25382
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression and developmental regulation of Ehk-1, a neuronal Elk-like receptor tyrosine kinase in brain.
Journal
Neuroscience
Author(s)
Taylor V., Miescher G.C., Pfarr S., Honegger P., Breitschopf H., Lassmann H., Steck A.J.
ISSN
0306-4522 (Print)
ISSN-L
0306-4522
Publication state
Published
Issued date
1994
Volume
63
Number
1
Pages
163-178
Language
english
Abstract
Protein tyrosine kinases are pivotal in central nervous tissue development and maintenance. Here we focus on the expression of Ehk-1, a novel Elk-related receptor tyrosine kinase. Ehk-1 gene expression is observed in the developing and adult central nervous system and is highly regulated throughout development at both the messenger RNA and protein levels. Three messenger RNA transcripts of 8.5, 5.9 and 5.1 kb are detectable in the rat brain and a variety of splice possibilities have been identified. However, a major protein species of around M(r) 120,000 predominates throughout development. Ehk-1 messenger RNA and protein levels are highest in the first postnatal week. By in situ messenger RNA hybridization the gene is expressed by all neurons of the adult brain, but mostly in the hippocampus, cerebral cortex and large neurons of the deep cerebellar nuclei, as well as the Purkinje and granular cells of the cerebellum. At earlier stages of development, transcripts are most prominent in the periventricular germinal layers of the brain. Immunohistochemistry reveals a pronounced membrane associated protein expression in immature neurons. In the adult animal, peak reactivity was found in the neuropil with sparing of most perikarya. The spatial and temporal pattern of ehk-1 gene expression suggests a role in both the development and maintenance of differentiated neurons of the central nervous system.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, Brain/cytology, Brain/enzymology, Cloning, Molecular, Gene Expression Regulation, Developmental/physiology, Immunohistochemistry, In Situ Hybridization, Molecular Sequence Data, Nerve Tissue Proteins/metabolism, Neurons/enzymology, Polymerase Chain Reaction, RNA, Messenger/biosynthesis, Rats, Rats, Sprague-Dawley, Receptor Protein-Tyrosine Kinases/biosynthesis, Receptor, EphA5, Transcription, Genetic
Pubmed
Web of science
Create date
24/01/2008 14:11
Last modification date
03/03/2018 15:26
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