Highly diverse TCRα chain repertoire of pre-immune CD8(+) T cells reveals new insights in gene recombination.

Details

Serval ID
serval:BIB_2B947DF61C00
Type
Article: article from journal or magazin.
Collection
Publications
Title
Highly diverse TCRα chain repertoire of pre-immune CD8(+) T cells reveals new insights in gene recombination.
Journal
Embo Journal
Author(s)
Genolet R., Stevenson B.J., Farinelli L., Osterås M., Luescher I.F.
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
31
Number
7
Pages
1666-1678
Language
english
Notes
Publication types: Journal Article
Abstract
Although the T-cell receptor αδ (TCRαδ) locus harbours large libraries of variable (TRAV) and junctional (TRAJ) gene segments, according to previous studies the TCRα chain repertoire is of limited diversity due to restrictions imposed by sequential coordinate TRAV-TRAJ recombinations. By sequencing tens of millions of TCRα chain transcripts from naive mouse CD8(+) T cells, we observed a hugely diverse repertoire, comprising nearly all possible TRAV-TRAJ combinations. Our findings are not compatible with sequential coordinate gene recombination, but rather with a model in which contraction and DNA looping in the TCRαδ locus provide equal access to TRAV and TRAJ gene segments, similarly to that demonstrated for IgH gene recombination. Generation of the observed highly diverse TCRα chain repertoire necessitates deletion of failed attempts by thymic-positive selection and is essential for the formation of highly diverse TCRαβ repertoires, capable of providing good protective immunity.
Pubmed
Web of science
Open Access
Yes
Create date
05/05/2012 15:33
Last modification date
20/08/2019 13:10
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