Treatment with 3-aminobenzamide during ex vivo lung perfusion of damaged rat lungs reduces graft injury and dysfunction after transplantation.

Details

Serval ID
serval:BIB_2B93F2051758
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Treatment with 3-aminobenzamide during ex vivo lung perfusion of damaged rat lungs reduces graft injury and dysfunction after transplantation.
Journal
American journal of transplantation
Author(s)
Wang X., Parapanov R., Debonneville A., Wang Y., Abdelnour-Berchtold E., Gonzalez M., Gronchi F., Perentes J.Y., Ris H.B., Eckert P., Piquilloud L., Lugrin J., Letovanec I., Krueger T., Liaudet L.
ISSN
1600-6143 (Electronic)
ISSN-L
1600-6135
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
20
Number
4
Pages
967-976
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Ex vivo lung perfusion (EVLP) with pharmacological reconditioning may increase donor lung utilization for transplantation (LTx). 3-Aminobenzamide (3-AB), an inhibitor of poly(ADP-ribose) polymerase (PARP), reduces ex vivo lung injury in rat lungs damaged by warm ischemia (WI). Here we determined the effects of 3-AB reconditioning on graft outcome after LTx. Three groups of donor lungs were studied: Control (Ctrl): 1 hour WI + 3 hours cold ischemia (CI) + LTx; EVLP: 1 hour WI + 3 hours EVLP + LTx; EVLP + 3-AB: 1 hour WI + 3 hours EVLP + 3-AB (1 mg <sup>.</sup> mL <sup>-1</sup> ) + LTx. Two hours after LTx, we determined lung graft compliance, edema, histology, neutrophil counts in bronchoalveolar lavage (BAL), mRNA levels of adhesion molecules within the graft, as well as concentrations of interleukin-6 and 10 (IL-6, IL-10) in BAL and plasma. 3-AB reconditioning during EVLP improved compliance and reduced lung edema, neutrophil infiltration, and the expression of adhesion molecules within the transplanted lungs. 3-AB also attenuated the IL-6/IL-10 ratio in BAL and plasma, supporting an improved balance between pro- and anti-inflammatory mediators. Thus, 3-AB reconditioning during EVLP of rat lung grafts damaged by WI markedly reduces inflammation, edema, and physiological deterioration after LTx, supporting the use of PARP inhibitors for the rehabilitation of damaged lungs during EVLP.
Keywords
adhesion molecules/integrins, animal models: murine, basic (laboratory) research/science, donors and donation: donor evaluation, lung failure/injury, lung transplantation/pulmonology, organ procurement and allocation, primary nonfunction, translational research/science
Pubmed
Web of science
Create date
22/10/2019 7:59
Last modification date
20/01/2021 19:30
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