In and out: Leishmania metastasis by hijacking lymphatic system and migrating immune cells.

Details

Serval ID
serval:BIB_2B104E1E8C7E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In and out: Leishmania metastasis by hijacking lymphatic system and migrating immune cells.
Journal
Frontiers in cellular and infection microbiology
Author(s)
Jha B., Reverte M., Ronet C., Prevel F., Morgenthaler F.D., Desponds C., Lye L.F., Owens K.L., Scarpellino L., Dubey L.K., Sabine A., Petrova T.V., Luther S.A., Beverley S.M., Fasel N.
ISSN
2235-2988 (Electronic)
ISSN-L
2235-2988
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
12
Pages
941860
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The lymphatic system plays a crucial role in mounting immune response against intracellular pathogens, and recent studies have documented its role in facilitating tumor dissemination linked largely with cancer cells. However, in mucocutaneous leishmaniasis (MCL) caused by Leishmania Viannia subgenus showing infectious metastasis and resulting in severe distant secondary lesions, the route of escape of these parasites to secondary sites has not yet been investigated in detail. Our results demonstrated that when infection was associated with inflammation and additionally exacerbated by the presence of dsRNA viral endosymbiont (LRV1), lymphatic vessels could serve as efficient routes for infected cells to egress from the primary site and colonize distant organs. We challenged this hypothesis by using the intracellular Leishmania protozoan parasites Leishmania guyanensis (Lgy) associated with or without a dsRNA viral endosymbiont, exacerbating the infection and responsible for a strong inflammatory response, and favoring metastasis of the infection. We analyzed possible cargo cells and the routes of dissemination through flow cytometry, histological analysis, and in vivo imaging in our metastatic model to show that parasites disseminated not only intracellularly but also as free extracellular parasites using migrating immune cells, lymph nodes (LNs), and lymph vessels, and followed intricate connections of draining and non-draining lymph node to finally end up in the blood and in distant skin, causing new lesions.
Keywords
Humans, Leishmania, Leishmania braziliensis, Leishmaniasis, Mucocutaneous, Lymphatic System, Neoplasms, Leishmania RNA virus 1 (LRV1), dissemination, extracellular, free amastigotes, inflammation, lymph nodes (LNs), metastasis
Pubmed
Open Access
Yes
Create date
05/09/2022 8:19
Last modification date
02/11/2022 6:41
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