Genetic and molecular characterization of the Pseudomonas plasmid pVS1.
Details
Serval ID
serval:BIB_2A94C090DF71
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genetic and molecular characterization of the Pseudomonas plasmid pVS1.
Journal
Plasmid
ISSN
0147-619X (Print)
ISSN-L
0147-619X
Publication state
Published
Issued date
1984
Volume
11
Number
3
Pages
206-220
Language
english
Abstract
A restriction map of the 30-kb nonconjugative Pseudomonas plasmid pVS1 was constructed. Derivatives of pVS1 obtained in vitro by successive deletions were used to localize on the physical map the determinant for resistance to mercuric ions (carried by transposon Tn501), the gene(s) encoding sulfonamide resistance, a 1.6-kb region affecting plasmid stability and establishment in P. fluorescens ATCC 13525, and a segment required for mobilization of pVS1 by plasmid RP1. The sulfonamide resistance determinant of pVS1 appeared to be closely related to that of transposon Tn21. A mini-pVS1 replicon, pME259, consisting of an essential 1.55-kb segment (designated rep and thought to carry the origin of replication) and a mercury resistance determinant was able to replicate P. aeruginosa PAO but selective pressure was needed for plasmid maintenance. The copy number of pVS1 derivatives was estimated to be 6-8 per chromosome equivalent. Plasmids possessing the essential rep segment plus the adjacent stability region could be established in strains of P. aeruginosa, P. putida, P. fluorescens, P. acidovorans, P. cepacia, P. mendocina, P. stutzeri, P. syringae, Agrobacterium tumefaciens, and Rhizobium leguminosarum.
Keywords
DNA Replication, DNA Restriction Enzymes, DNA, Bacterial/genetics, Drug Resistance, Microbial, Genetic Vectors, Mercury/pharmacology, Plasmids, Pseudomonas/drug effects, Pseudomonas/genetics, Species Specificity, Sulfonamides/pharmacology
Pubmed
Web of science
Create date
25/01/2008 17:01
Last modification date
20/08/2019 13:10