Overproduction of the bleomycin-binding proteins from bleomycin-producing Streptomyces verticillus and a methicillin-resistant Staphylococcus aureus in Escherichia coli and their immunological characterisation.

Details

Serval ID
serval:BIB_2A5C853C7CA8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Overproduction of the bleomycin-binding proteins from bleomycin-producing Streptomyces verticillus and a methicillin-resistant Staphylococcus aureus in Escherichia coli and their immunological characterisation.
Journal
FEBS letters
Author(s)
Sugiyama M., Kumagai T., Matsuo H., Bhuiyan M.Z., Ueda K., Mochizuki H., Nakamura N., Davies J.E.
ISSN
0014-5793 (Print)
ISSN-L
0014-5793
Publication state
Published
Issued date
27/03/1995
Peer-reviewed
Oui
Volume
362
Number
1
Pages
80-84
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The bleomycin-binding proteins designated BLMA and BLMS, which confer resistance to bleomycin (Bm), from Bm-producing Streptomyces verticillus ATCC15003 and a methicillin-resistant Staphylococcus aureus B-26, respectively, were overexpressed in Escherichia coli. The present study showed that both BLMA and BLMS quench the antibacterial activity of Bm by the binding to the drug. To immuno-characterize the Bm-binding proteins, we constructed a monoclonal antibody against BLMA. The antibody, designated 893-12, did not cross react to BLMS and another Bm-binding protein from tallysomycin-producing Streptoalloteichus hindustanus. Although the ability of Bm to cleavage DNA was eliminated by a binding of BLMA to Bm, as shown by Sugiyama et al. [Gene 151 (1994) 11-15], the Bm-induced DNA degradation was restored by pre-incubation of BLMA with the anti-BLMA monoclonal antibody.

Keywords
Acetyltransferases, Amino Acid Sequence, Antibodies, Monoclonal/immunology, Bacterial Proteins/biosynthesis, Bacterial Proteins/genetics, Bacterial Proteins/immunology, Bacterial Proteins/metabolism, Base Sequence, Bleomycin/biosynthesis, Bleomycin/metabolism, Bleomycin/pharmacology, Blotting, Western, Carrier Proteins/biosynthesis, Carrier Proteins/genetics, Carrier Proteins/immunology, Carrier Proteins/metabolism, Escherichia coli/metabolism, Isoelectric Point, Methicillin Resistance, Molecular Sequence Data, Promoter Regions, Genetic, Recombinant Proteins/biosynthesis, Staphylococcus aureus/drug effects, Staphylococcus aureus/metabolism, Streptomyces/metabolism
Pubmed
Web of science
Create date
01/03/2018 15:43
Last modification date
20/08/2019 13:10
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