Neurotensin is a regulator of insulin secretion in pancreatic beta-cells.
Details
Serval ID
serval:BIB_2A55CC343481
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Neurotensin is a regulator of insulin secretion in pancreatic beta-cells.
Journal
International Journal of Biochemistry and Cell Biology
ISSN
1878-5875[electronic], 1357-2725[linking]
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
42
Number
10
Pages
1681-1688
Language
english
Abstract
Neurotensin (NT) is secreted from neurons and gastrointestinal endocrine cells. We previously reported that the three NT receptors (NTSRs) are expressed in pancreatic islets and beta cell lines on which we observed a protective effect of NT against cytotoxic agents. In this study, we explored the role of NT on insulin secretion in the endocrine pancreatic beta cells. We observed that NT stimulates insulin secretion at low glucose level and has a small inhibiting effect on stimulated insulin secretion from isolated islets or INS-1E cells. We studied the mechanisms by which NT elicited calcium concentration changes using fura-2 loaded islets or INS-1E cells. NT increases calcium influx through the opening of cationic channels. Similar calcium influxes were observed after treatment with NTSR selective ligands. NT-evoked calcium regulation involves PKC and the translocation of PKCalpha and PKCepsilon to the plasma membrane. Part of NT effects appears to be also mediated by PKA but not via the Erk pathway. Taken together, these data provide evidence for an important endocrine role of NT in the regulation of the secretory function of beta cells.
Keywords
Pancreatic islet, Neurotensin, PKC, Insulin secretion
Pubmed
Web of science
Create date
19/10/2010 7:00
Last modification date
20/08/2019 13:10