Isoelectric focusing of alpha-1 acid glycoprotein (orosomucoid) in immobilized pH-gradients with 8M urea: detection of its desialylated variants using an alkaline phosphatase-linked secondary antibody system.
Details
Serval ID
serval:BIB_2A23F6FF4D1E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isoelectric focusing of alpha-1 acid glycoprotein (orosomucoid) in immobilized pH-gradients with 8M urea: detection of its desialylated variants using an alkaline phosphatase-linked secondary antibody system.
Journal
Electrophoresis
ISSN
0173-0835 (Print)
ISSN-L
0173-0835
Publication state
Published
Issued date
1988
Peer-reviewed
Oui
Volume
9
Number
10
Pages
650-654
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
A method allowing a clear separation of the different variants of desialylated alpha 1-acid glycoprotein (orosomucoid) has been developed using isoelectric focusing in immobilized pH gradients, supplemented with 8 M urea and 2% v/v 2-mercaptoethanol. Immunoblotting with two antibody-steps afforded high sensitivity and permitted the detection of about 700 pg of alpha 1-acid glycoprotein in a 20 microL plasma sample diluted 1:28 672. A one year old bloodstrain, kept at room temperature, could easily be phenotyped.
Keywords
Alkaline Phosphatase, Blood Stains, Genetic Variation, Humans, Hydrogen-Ion Concentration, Immunoblotting, Isoelectric Focusing/methods, Neuraminidase, Orosomucoid/genetics, Orosomucoid/isolation & purification, Phenotype, Urea
Pubmed
Web of science
Create date
01/03/2013 10:00
Last modification date
20/08/2019 13:09
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