The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues.
Details
Serval ID
serval:BIB_29A077DA3CE1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues.
Journal
Science advances
ISSN
2375-2548 (Electronic)
ISSN-L
2375-2548
Publication state
Published
Issued date
27/09/2024
Peer-reviewed
Oui
Volume
10
Number
39
Pages
eadq3352
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Brassinosteroid signaling is essential for plant growth as exemplified by the dwarf phenotype of loss-of-function mutants in BRASSINOSTEROID INSENSITIVE 1 (BRI1), a ubiquitously expressed Arabidopsis brassinosteroid receptor gene. Complementation of brassinosteroid-blind receptor mutants by BRI1 expression with various tissue-specific promoters implied that local brassinosteroid signaling may instruct growth non-cell autonomously. Here, we performed such rescues with a panel of receptor variants and promoters, in combination with tissue-specific transgene knockouts. Our experiments demonstrate that brassinosteroid receptor expression in several tissues is necessary but not sufficient for rescue. Moreover, complementation with tissue-specific promoters requires the genuine BRI1 gene body sequence, which confers ubiquitous expression of trace receptor amounts that are sufficient to promote brassinosteroid-dependent root growth. Our data, therefore, argue for a largely cell-autonomous action of brassinosteroid receptors.
Keywords
Brassinosteroids/metabolism, Arabidopsis Proteins/metabolism, Arabidopsis Proteins/genetics, Signal Transduction, Arabidopsis/metabolism, Arabidopsis/genetics, Gene Expression Regulation, Plant, Protein Kinases/metabolism, Protein Kinases/genetics, Promoter Regions, Genetic, Plants, Genetically Modified, Plant Roots/metabolism, Plant Roots/growth & development, Mutation, Organ Specificity/genetics
Pubmed
Open Access
Yes
Create date
27/09/2024 15:46
Last modification date
28/09/2024 7:09