Optogenetic activators of apoptosis, necroptosis, and pyroptosis.

Details

Serval ID
serval:BIB_2987155B2350
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Optogenetic activators of apoptosis, necroptosis, and pyroptosis.
Journal
The Journal of cell biology
Author(s)
Shkarina K., Hasel de Carvalho E., Santos J.C., Ramos S., Leptin M., Broz P.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
06/06/2022
Peer-reviewed
Oui
Volume
221
Number
6
Pages
e202109038
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Targeted and specific induction of cell death in an individual or groups of cells hold the potential for new insights into the response of tissues or organisms to different forms of death. Here, we report the development of optogenetically controlled cell death effectors (optoCDEs), a novel class of optogenetic tools that enables light-mediated induction of three types of programmed cell death (PCD)-apoptosis, pyroptosis, and necroptosis-using Arabidopsis thaliana photosensitive protein Cryptochrome-2. OptoCDEs enable a rapid and highly specific induction of PCD in human, mouse, and zebrafish cells and are suitable for a wide range of applications, such as sub-lethal cell death induction or precise elimination of single cells or cell populations in vitro and in vivo. As the proof-of-concept, we utilize optoCDEs to assess the differences in neighboring cell responses to apoptotic or necrotic PCD, revealing a new role for shingosine-1-phosphate signaling in regulating the efferocytosis of the apoptotic cell by epithelia.
Keywords
Animals, Apoptosis/genetics, Arabidopsis/genetics, Cryptochromes/genetics, Humans, Lysophospholipids/metabolism, Mice, Necroptosis/genetics, Optogenetics, Pyroptosis/genetics, Sphingosine/analogs & derivatives, Sphingosine/metabolism, Zebrafish/genetics
Pubmed
Create date
25/04/2022 11:53
Last modification date
03/05/2022 6:35
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